CELLview cell culture dish, four compartments, Advanced TC treated, Sterile, Case of 40
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Product code: 627975
MPN: 627975
Manufacturer: Greiner Bio-One
Shipping Weight: 0.88lbs (0.40kg)
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Description
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Packaging
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Detailed information
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CELLview - Cell Culture Dish with Glass Bottom
Drug treatment during live cell imagingย
A multi-position time-lapse experiment was started and after acquiring six time points every two minutes drugs were added to the different wells as indicated:
| Description: |
CELLVIEW CELL CULTURE DISH, PS, 35/10 MM, GLASS BOTTOM, 4 COMPARTMENTS, ADVANCED TC, STERILE, 10 PCS./BAG |
|---|---|
| Sterile: | sterile |
| Packing unit: | 40 |
| Qty / inner pack: | 10 |
Packaging
| Packaging weight: | 0.40 kg |
|---|---|
| Packaging dimension: | 195 x 142 x 122 mm |
| Packing unit: | 40 |
| Qty / inner pack: | 10 |
| PAL: | 22800 |
Detailed information
| Filling volume (ml): | 0.00 |
|---|
CELLview - Cell Culture Dish with Glass Bottom
- Free of detectable DNase, RNase, human DNA
- Non-pyrogenic, non-cytotoxic
- Glass bottom features:
- High transparent achromatic borosilicate glass; hydrolytic>- Glass thickness 175 ยตm +/- 15 ยตm
- Maximal spectral transmission; no autofluorescenceย
- Advantages:
-Subdivided version enables simultaneous multiplex analysis
- Embedded glass bottom for maximal planarity
- Number of compartments: 4
- Diameter: 35 mm; height: 10 mm
- Growth area: 1.9 cmยฒ/compartment
- Total volume: 1.5 ml/compartment
- Working volume: 0.1 ml for seeding or staining only on glass area; 0.5 ml for cultivation in the complete compartment
- Surface treatment: Advanced TCย
- Sterile
Drug treatment during live cell imagingย
A multi-position time-lapse experiment was started and after acquiring six time points every two minutes drugs were added to the different wells as indicated:
- Video 1ย - control (no drugs added)
In steady-state the Golgi apparatus is relatively stable on light microscopy level. The shape changes only slowly during the time of the experiment when observing control cells. Also the number of Golgi fragments visible by light microscopy resolution is relatively constant over time.
- Video 2ย - Nocodazole added, final concentration 10 ยตM
Nocodazole treatment induces, fragmentation of the Golgi apparatus. The onset of fragmentation starts 10 to 15 minutes after addition of the drug. The onset of fragmentation differs between individual cells. Fragmentation of the central Golgi to many distributed ministacks is the final phenotype of microtubule depolymerization after three hours.
- Video 3ย - Latrunculin B added, final concentration 1 ฮผM
Actin depolymerization by Latrunculin B influences the shape of the Golgi from relatively thin elongated to a rounded up and compact appearance. After 10 to 20 minutes differences in the Golgi morphology became first visible and after approximately one hour the Golgi rearrangement was completed.
- Video 4ย - Brefeldin A added, final concentration 5 ฮผg/ml
Block of export from the endoplasmatic reticulum (ER) by Brefeldin A leads to a rapid redistribution of the Golgi compartment to the ER by retrograde transport. This effect is often completed within 5 minutes.
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