Diazyme Enzymatic Sodium Two Level Calibrator Set

Diazyme Enzymatic Sodium Two Level Calibrator Set

Enzymatic Sodium Two (2) Level Calibrator Set (liquid), Cal: 2 x 3 mL vials

Diazyme's Sodium Assay has good correlation with the ISE method and has a linear method between sodium concentrations of 80 and 180 mmole/L (184 and 414  mg/dL). The assay has CV's% of <2% with a correlation coefficient of 0.98, slope of 1.05 and a y intercept of -2.23. The following substances normally present in  serum produced less than 10% deviation at the listed concentrations: NH4Cl at 1.5 mM, KPi at 2.0 mM, CaCl2 at 7.5 mM, KCl at 10 mM, CuCl2 at 0.5 mM, ZnCl2 at 0.5 mM, FeCl3 at 0.5 mM, Glucose at 5 mM, ascorbate 10 mM, bilirubin at 40 mg/dL, bilirubin conjugate 40 mg/dL, hemoglobin 500 mg/dL, and triglyceride  1000 mg/dL.

Assay Principle

Sodium is determined enzymatically via sodium-dependent β- galactosidase activity with  ONPG as the substrate. The absorbance at 405 nm of the product O-nitrophenyl is proportional  to the sodium concentration.

Intended Use

Diazyme Liquid Stable Enzymatic Sodium Assay is intended for the quantitative in vitro determination of sodium in serum. Measurements obtained by this device  are used in the diagnosis and treatment of aldosteronism (excessive secretion of the hormone aldosterone), diabetes insipidus (chronic excretion of large amounts  of dilute urine, accompanied by extreme thirst), adrenal hypertension, Addison's disease (caused by destruction of the adrenal glands), dehydration, inappropriate  antidiuretic hormone secretion, or other diseases involving electrolyte imbalance.