For Business Use Only. Does Not Ship to Residential Addresses. For use inside an Analyzer, Sold Separately.
Diazyme 5 Nucleotidase Calibrator
- Get your quote in 90 minutes.
- Trade in your older analyzers to recover budget.
- Bundle equipment, reagents, & consumables into one cost-saving order.
- Enlist our local service tech to keep uptime high.
- Lower your cost per test.
Meet your sales rep Ben Morton, covering your area.
Would you like to book 15 minutes on Ben Morton's calendar to get the best value for your budget?
Yes, book the timeIn Stock
Product code: DZ123A-CAL
MPN: DZ123A-CAL
Manufacturer: Diazyme
Shipping Weight: 5.00lbs (2.27kg)
Diazyme 5 Nucleotidase Calibrator
5' - Nucleotidase Calibrator (lyophilized), Cal: 1 x 1 mL vial
Diazyme's 5'-Nucleotidase Assay is linear from 0 - 300 U/L (R² >0.99) with intra-assay CV% of < 2% and an inter-assay CV% of ≤ 4%. 5'-Nucleotidase assay is not affected by interfering substances such as serum bilirubin up to 40 mg/dL, hemoglobin up to 500 mg/dL, triglycerides up to 1250 mg/dL, ascorbic acid up to 20 mg/dL, and alkaline phosphatase up to 1250 U/L.
Assay Principle
The 5'-NT assay is based on the enzymatic hydrolysis of 5'-monophosphate (5'-IMP) to form inosine which is converted to hypoxanthine by purine nucleoside phosphorylase (PNP). Hypoxanthine is then converted to uric acid and hydrogen peroxide (H2O2) by xanthine oxidase (XOD). H2O2 is further reacted with N-Ethyl- N-(2-hydroxy-3-sulfopropyl)-3-methylaniline (EHSPT) and 4- aminoantipyrine (4-AA) in the presence of peroxidase (POD) to generate quinone dye which is monitored in a kinetic manner.
Intended Use
The 5'-Nucleotidase (5'-NT) assay kit is for the determination of 5'-NT activity in human serum samples.
