varicella zoster iga elisa, 96 wells
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- drg international inc.
- varicella zoster iga elisa, 96 wells
a leading provider of laboratory equipment and supplies, block scientific offers varicella zoster iga elisa, 96 wells at competitive prices. serving the lab equipment market for many years, block scientific is a global company providing a wide range of infectious disease kits for diagnostic and research purposes.
enzyme immunoassay kit
– solid phase elisa
the drg varicella zoster virus (vzv) iga elisa kit is a solid phase enzyme-linked immunosorbent assay (elisa). it provides materials for the qualitative and semiquantitative determination of iga-class antibodies to varicella zoster virus in serum.
patient samples are diluted with sample diluent and additionally incubated with igg-rf-sorbent, containing hyperimmune anti-human igg-class antibody to eliminate competitive inhibition from specific igg and to remove rheumatoid factors. this pretreatment avoids false negative or false positive results.
microtiter wells as a solid phase are coated with varicella zoster virus antigen.
diluted patient specimens and ready-for-use controls are pipetted into these wells. during incubation varicella zoster virus-specific antibodies of positive specimens and controls are bound to the immobilized antigens.
after a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human iga antibodies are dispensed into the wells. during a second incubation this anti-iga conjugate binds specifically to iga antibodies resulting in the formation of enzyme-linked immune complexes.
after a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with tmb substrate and development of a blue color. the blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid.
the intensity of this color is directly proportional to the amount of varicella zoster virus-specific iga antibody in the patient specimen. absorbance at 450 nm is read using an elisa microtiter plate reader.
this kit is intended for research use only
not for use in diagnostic procedures
- kit components – microtiterwells, igg-rf-sorbent, sample diluent, pos. control, neg. control, enzyme conjugate, cut-off control, stop solution, substrate solution, wash solution
- storage – when stored at 2 °c to 8 °c unopened reagents will retain reactivity until expiration date
if you have any questions about the varicella zoster iga elisa, 96 wells or other infectious disease kits, dial 631-589-1118 and speak to one of our customer service representatives. alternatively, you can send an email to email@example.com.