Ureaplasma Urealyticum IgG ELISA, 96 wells
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Manufacturer: DRG International Inc.
Model: Ureaplasma Urealyticum IgG ELISA, 96 wells
Product Code: EIA4623
Block Scientific offers Ureaplasma urealyticum IgG ELISA, 96 wells as well as other kinds of infectious disease kits from the well known manufacturer – DRG International Inc. We are one of the prominent lab equipment dealers in the US, supplying brand name products from reliable manufacturers in the industry.
Enzyme Immunoassay Kit
– Solid Phase ELISA
The DRG Ureaplasma urealyticum IgG ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA). It provides materials for the qualitative and semiquantitative determination of IgG-class antibodies to Ureaplasma urealyticum in serum.
The DRG Ureaplasma urealyticum IgG ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) Microtiter wells as a solid phase are coated with Ureaplasma urealyticum antigen.
Diluted patient specimens and ready-for-use controls are pipetted into these wells. During incubation Ureaplasma urealyticum-specific antibodies of positive specimens and controls are bound to the immobilized antigens.
After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgG antibodies are dispensed into the wells. During a second incubation this anti-IgG conjugate binds specificly to IgG antibodies resulting in the formation of enzyme-linked immune complexes.
After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid.
The intensity of this color is directly proportional to the amount of Ureaplasma urealyticum-specific IgG antibody in the patient specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.
- Kit Components – Microtiterwells, pos. control, low control, sample diluent, calibrator, substrate solution, enzyme conjugate, wash solution, stop solution
- Storage – When stored at 2 °C to 8 °C unopened reagents will retain reactivity until expiration date
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