tuberculosis igg elisa, 96 wells
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- drg international inc.
- tuberculosis igg elisa, 96 wells
block scientific is an established laboratory equipment supplier in the united states that has earned a reputation as a reliable source for a range of important laboratory apparatuses. among the products we distribute is the tuberculosis igg elisa, 96 wells required for various research applications.
tuberculosis igg elisa
– quality assay
drg tuberculosis elisa is a quality assay designed for the detection of igg / igm / iga antibodies against mycobacterium tuberculosis in serum and plasma. further applications in other body fluids are possible and can be provided on request.
the principle of the test reaction can be described in four stages.
- serum incubation
- conjugate incubation
- substrate reaction and stopping
- reading and interpretation
specific antibodies bind to the antigens on the solid phase to form a stable immune complex. after a 60 minutes incubation at room temperature the wells are washed with prediluted wash buffer to remove all nonreactive serum components.
the anti-human-igg /-igm /-iga horseradish peroxidase conjugate is added to all wells. the conjugate binds to igg / igm / iga antibodies on the solid phase antigen to form a stable sandwich. after a 30 minutes incubation at room temperature the excess conjugate is removed by washing all wells with washing buffer.
the tmb substrate is dispensed into each well and the peroxidase enzyme/substrate reaction forms a stable blue chromogen. the reaction and subsequently the colour development is stopped after 20 minutes incubation at room temperature by adding 0.5 m h2so4 to the wells. the change in ph also causes the chromogen to change colour from blue to yellow.
the intensity of the colour is read in a microtiter plate reader at 450 nm (recommended reference wavelength for bichromatic measurement: 600-690 nm). the intensity of the colour (od) is directly proportional to the concentration of the specific antibody in the donor sample.
intended for research use only
- kit contents – microtiter strips, standards 1-4, frame holder, enzyme conjugate, serum diluent, stop solution, wash buffer, tmb substrate solution, plastic bag, plate sealers
- storage – strips and solutions can be stored at 2-8°c
for more information on the tuberculosis igg elisa, 96 wells, dial 631-589-1118 or send an e-mail to firstname.lastname@example.org. to view other infectious disease kits that we deal in, please visit our online store.