buy now

tuberculosis iga elisa, 96 wells

to add this product to your interest list, sign up or login.

tuberculosis iga elisa, 96 wells
  1. drg international inc.
  2. tuberculosis iga elisa, 96 wells
  3. eia4250

block scientific offers tuberculosis iga elisa, 96 wells as well as other kinds of infectious disease kits from the well known manufacturer – drg international inc. we are one of the prominent lab equipment dealers in the us, supplying brand name products from reliable manufacturers in the industry.

tuberculosis iga elisa test kit

– quality elisa

the drg mumps virus igm elisa kit is a solid phase enzyme-linked immunosorbent assay (elisa). it provides materials for the qualitative and semiquantitative determination of igm-class antibodies to mumps virus in serum.

principle of the test

the principle of the test reaction can be described in four stages.

  • serum incubation
  • specific antibodies bind to the antigens on the solid phase to form a stable immune complex. after a 60 minutes incubation at room temperature the wells are washed with prediluted wash buffer to remove all nonreactive serum components.

  • conjugate incubation
  • the anti-human-igg /-igm /-iga horseradish peroxidase conjugate is added to all wells. the conjugate binds to igg / igm / iga antibodies on the solid phase antigen to form a stable sandwich. after a 30 minutes incubation at room temperature the excess conjugate is removed by washing all wells with washing buffer.

  • substrate reaction and stopping
  • the tmb substrate is dispensed into each well and the peroxidase enzyme/substrate reaction forms a stable blue chromogen. the reaction and subsequently the colour development is stopped after 20 minutes incubation at room temperature by adding 0.5 m h2so4 to the wells. the change in ph also causes the chromogen to change colour from blue to yellow.

  • reading and interpretation
  • the intensity of the colour is read in a microtiter plate reader at 450 nm (recommended reference wavelength for bichromatic measurement: 600-690 nm). the intensity of the colour (od) is directly proportional to the concentration of the specific antibody in the donor sample.


  • kit components – microtiterwells, igg rf sorbent***, sample diluent, negative control, positive control, enzyme conjugate, cut-off control, stop solution, substrate solution, wash solution
  • storage – strips and solutions can be stored at 2 °c to 8 °c.

browse our online store to check out other infectious disease kits. if you have any queries about the tuberculosis iga elisa, 96 wells, you can contact us by phone: 631-589-1118, or e-mail: