tuberculosis iga elisa, 96 wells
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- drg international inc.
- tuberculosis iga elisa, 96 wells
block scientific offers tuberculosis iga elisa, 96 wells as well as other kinds of infectious disease kits from the well known manufacturer – drg international inc. we are one of the prominent lab equipment dealers in the us, supplying brand name products from reliable manufacturers in the industry.
tuberculosis iga elisa test kit
– quality elisa
the drg mumps virus igm elisa kit is a solid phase enzyme-linked immunosorbent assay (elisa). it provides materials for the qualitative and semiquantitative determination of igm-class antibodies to mumps virus in serum.
principle of the test
the principle of the test reaction can be described in four stages.
- serum incubation
- conjugate incubation
- substrate reaction and stopping
- reading and interpretation
specific antibodies bind to the antigens on the solid phase to form a stable immune complex. after a 60 minutes incubation at room temperature the wells are washed with prediluted wash buffer to remove all nonreactive serum components.
the anti-human-igg /-igm /-iga horseradish peroxidase conjugate is added to all wells. the conjugate binds to igg / igm / iga antibodies on the solid phase antigen to form a stable sandwich. after a 30 minutes incubation at room temperature the excess conjugate is removed by washing all wells with washing buffer.
the tmb substrate is dispensed into each well and the peroxidase enzyme/substrate reaction forms a stable blue chromogen. the reaction and subsequently the colour development is stopped after 20 minutes incubation at room temperature by adding 0.5 m h2so4 to the wells. the change in ph also causes the chromogen to change colour from blue to yellow.
the intensity of the colour is read in a microtiter plate reader at 450 nm (recommended reference wavelength for bichromatic measurement: 600-690 nm). the intensity of the colour (od) is directly proportional to the concentration of the specific antibody in the donor sample.
- kit components – microtiterwells, igg rf sorbent***, sample diluent, negative control, positive control, enzyme conjugate, cut-off control, stop solution, substrate solution, wash solution
- storage – strips and solutions can be stored at 2 °c to 8 °c.
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