TSH, Ultra, 96 Wells

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TSH, Ultra, 96 Wells

Manufacturer: DRG International Inc.
Model: TSH, ultra, 96 Wells
Product Code: EIA-1790

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Elisa Kit

– Quality Enzyme Immunoassay

This product is a quality enzyme immunoassay for the quantitative determination of thyroid stimulating hormone (TSH) concentration in human serum. It is useful in the diagnosis of thyroid or pituitary disorders.


The determination of serum or plasma levels of thyroid stimulating hormone (TSH or thyrotropin) is recognized as a sensitive method in the diagnosis of primary and secondary hyp othyroidism. (1) TSH is secreted by the anterior lobe of the pituitary gland and induces the production and release of thyroxine and triiodothyronine from the thyroid gland.(2) It is a glycoprotein with a molecular weight of approximately 28,000 daltons, consisting of two chemically different subunits, alpha and beta.(3)

Although the concentration of TSH in the blood is extremely low, it is essential for the maintenance of normal thyroid function. The release of TSH is regulated by a TSH-releasing hormone (TRH) produced by the hypothalamus. The levels of TSH and TRH are inversely related to the level of thyroid hormone. When there is a high level of thyroid hormone in the blood, less TRH is released by the hypothalamus, so less TSH is secreted by the pituitary. The opposite action will occur when there is decreased thyroid hormone in the blood. This process is known as a negative feedback mechanism and is responsible for maintaining the proper blood levels of these hormones. (4,5)

TSH and the pituitary glycoproteins: luteinizing hormone (LH), follicle-stimulating hormone (FSH), and human chorionic gonadotropin (hCG), have identical alpha chains. The beta chains are distinct but do contain regions with identical amino acid sequences. These regions of homology can cause considerable cross-reactivity with some polyclonal TSH antisera. The use of a monoclonal antibody in this TSH ELISA test eliminates such cross-reactivity, which could result in falsely elevated TSH values in either menopausal or pregnant females – a population whose evaluation of thyroid status is clinically significant.(6,7,8).



  • Reagents and Materials Provided – Enzyme conjugate reagent (1 vial, 13 ml), antibody-coated wells (1 plate, 96 wells), reference standard set (1 ml/vial), stop solution (1N HCL) (1 bottle 11 ml), TMB reagent (1 bottle, 11 ml)
  • Storage – Can be stored at 2°C to 8°C.

For more information on TSH, 96 Wells and the range of other hormone assays that we carry, visit our online store. We are available on phone at 631-589-1118 or by e-mail at info@blockscientific.com.