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toxoplasma gondii igm elisa*, 96 wells

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toxoplasma gondii igm elisa*, 96 wells
  1. drg international inc.
  2. toxoplasma gondii igm elisa*, 96 wells
  3. eia3520

if you are in search of infectious disease kits to meet your routine assay requirements, then block scientific can make it available for you. as a well known lab equipment dealer with many years experience in the industry, block scientific has always focused on meeting even the most challenging client requirements. we offer the toxoplasma gondii igm elisa*, 96 wells at an affordable price.

enzyme immunoassay kit

– solid phase elisa

the drg toxoplasma gondii igm elisa kit is a solid phase enzyme-linked immunosorbent assay (elisa). it is suitable for determination of igm-class antibodies to toxoplasma gondii in serum.

test principle

samples are diluted with sample diluent and additionally incubated with igg-rf-sorbent, containing hyper-immune antihuman igg-class antibody to eliminate competitive inhibition from specific igg and to remove rheumatoid factors. this pretreatment avoids false negative or false positive results.

microtiter wells as a solid phase are coated with detergent treated toxoplasma gondii antigen (deelen strain). pretreated specimens, ready-for-use standards and ready-for-use control are pipetted into these wells. during incubation toxoplasma gondii-specific antibodies of positive specimens, and standards are bound to the immobilized antigens.

after a washing step to remove unbound sample, standard and control material horseradish peroxidase conjugated antihuman igm antibodies are dispensed into the wells. during a second incubation this anti-igm conjugate binds specifically to igm antibodies resulting in the formation of enzyme-linked immune complexes.

after a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with tmb substrate and development of a blue color. the blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid.

the intensity of this color is directly proportional to the amount of toxoplasma gondii-specific igm antibody in the specimen. absorbance at 450 nm is read using an elisa microtiter plate reader.


for research use only

not for use in diagnostic procedures

  • reagents provided – sample diluent, microtiterwells, igg-rf-sorbent, neg. control, pos. control, standard (standard 1-3), substrate solution, stop solution, enzyme conjugate, wash solution
  • storage – when stored at 2 °c to 8 °c unopened reagents will retain reactivity until expiration date

to know more

for more information about the toxoplasma gondii igm elisa*, 96 wells and other infectious disease kits, please visit our online store. for your queries, call 631-589-1118 or send an email to