toxoplasma gondii iga elisa*, 96 wells
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- drg international inc.
- toxoplasma gondii iga elisa*, 96 wells
block scientific offers toxoplasma gondii iga elisa*, 96 wells as well as other kinds of infectious disease kits from the well known manufacturer – drg international inc. we are one of the prominent lab equipment dealers in the us, supplying brand name products from reliable manufacturers in the industry.
enzyme immunoassay kit
– solid phase elisa
the drg toxoplasma gondii iga elisa kit is a solid phase enzyme-linked immunosorbent assay (elisa).
microtiter wells as a solid phase are coated with inactivated toxoplasma gondii antigen.
diluted patient specimens and ready-for-use controls are pipetted into these wells. during incubation toxoplasma gondii-specific antibodies of positive specimens and controls are bound to the immobilized antigens.
after a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human iga antibodies are dispensed into the wells. during a second incubation this anti-iga conjugate binds specifically to iga antibodies resulting in the formation of enzyme-linked immune complexes.
after a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with tmb substrate and development of a blue color. the blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid.
the intensity of this color is directly proportional to the amount of toxoplasma gondii-specific iga antibody in the patient specimen. absorbance at 450 nm is read using an elisa microtiter plate reader.
- reagents provided – microtiterwells, igg-rf-sorbent, sample diluent, pos. control, neg. control, enzyme conjugate, substrate solution, cut-off control, stop solution, wash solution
- storage – when stored at 2 °c to 8 °c unopened reagents will retain reactivity until expiration date.
browse our online store to check out other infectious disease kits. if you have any queries about the toxoplasma gondii iga elisa*, 96 wells, you can contact us by phone: 631-589-1118, or e-mail: email@example.com.