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tetanus toxin (antitoxin) igg elisa, 96 wells

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tetanus toxin (antitoxin) igg elisa, 96 wells
  1. drg international inc.
  2. eia3514

a leading provider of laboratory equipment and supplies, block scientific offers tetanus toxin (antitoxin) igg elisa, 96 wells at competitive prices. serving the lab equipment market for many years, block scientific is a global company providing a wide range of infectious disease kits for diagnostic and research purposes.

igg enzyme immunoassay kit

– quality elisa

the drg treponema pallidum igg elisa kit is a quality elisa for determination of igg-class antibodies to tetanus toxin in serum.

test principle

the drg tetanus toxin igg elisa kit is a solid phase enzyme-linked immunosorbent assay (elisa).

microtiter wells as a solid phase are coated with tetanus toxoid antigen.

diluted specimens and ready-for-use controls are pipetted into these wells. during incubation tetanus toxin-specific antibodies of positive specimens and controls are bound to the immobilized antigens.

after a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human igg antibodies are dispensed into the wells. during a second incubation this anti-igg conjugate binds specifically to igg antibodies resulting in the formation of enzyme-linked immune complexes.

after a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with tmb substrate and development of a blue color. the blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid.

the intensity of this color is directly proportional to the amount of tetanus toxin-specific igg antibody in the specimen.

absorbance at 450 nm is read using an elisa microtiter plate reader.


  • kit contents – microtiterwells, standard (1-3), sample diluent, low control, high control, substrate solution, sample diluent, wash solution, stop solution
  • storage – when stored at 2 °c to 8 °c unopened reagents will retain reactivity until expiration date

if you have any questions about the tetanus toxin (antitoxin) igg elisa, 96 wells or other infectious disease kits, dial 631-589-1118 and speak to one of our customer service representatives. alternatively, you can send an email to