syphilis (treponema) trep screen elisa, 96 wells
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- drg international inc.
a leading provider of laboratory equipment and supplies, block scientific offers syphilis (treponema) trep screen elisa, 96 wells at competitive prices. serving the lab equipment market for many years, block scientific is a global company providing a wide range of infectious disease kits for diagnostic and research purposes.
immunoassay kit – solid phase elisa
the drg treponema pallidum screen enzyme immunoassay kit provides materials for the qualitative determination of antibodies to treponema pallidum in serum or plasma.
the drg treponema pallidum screen elisa kit is a solid phase enzyme-linked immunosorbent assay (elisa).
microtiter wells as a solid phase are coated with specific, recombinant treponemal antigens.
patient specimens and ready-for-use controls are pipetted into these wells. during incubation treponema pallidumspecific antibodies of positive specimens and controls are bound to the immobilized antigens.
after a washing step to remove unbound sample and control material horseradish peroxidase conjugated treponemal antigens are dispensed into the wells. during a second incubation this conjugate binds specifically to antibodies resulting in the formation of enzyme-linked immune complexes.
after a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with tmb substrate and development of a blue color. the blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid.
the intensity of this color is directly proportional to the amount of treponema pallidum-specific antibody in the patient specimen. absorbance at 450 nm is read using an elisa microtiter plate reader.
for in vitro use only
- kit components – microplate, calibrator, positive control, negative control, specimen diluent, enzyme conjugate, wash buffer concentrate, chromogen/substrate, plate sealers, sulphuric acid
- a study conducted on an opened kit has not pointed out any relevant loss of activity up to 6 re-uses of the device and up to 3 months.
if you have any questions about the syphilis (treponema) trep screen elisa, 96 wells or other infectious disease kits that we carry, dial 631-589-1118 and speak to one of our customer service representatives. alternatively, you can send an email to email@example.com.