syphilis (treponema) trep screen (20 µl sample) elisa, 96 wells
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- drg international inc.
looking for syphilis (treponema) trep screen (20 μl sample) elisa, 96 wells from drg international inc? then approach block scientific. a leading lab apparatus distributor in new york with years of experience in the field, block scientific supplies a wide variety of consumables and accessories. we stock both new and refurbished lab equipment.
enzyme immunoassay kit
– solid phase elisa
the herpes simplex 1+2 igg elisa, 96 wells is a solid phase enzyme-linked immunosorbent assay (elisa). it provides materials for the qualitative determination of antibodies to treponema pallidum in serum or plasma.
microtiter wells as a solid phase are coated with specific, recombinant treponemal antigens.
patient specimens and ready-for-use controls are pipetted into these wells. during incubation treponema pallidumspecific antibodies of positive specimens and controls are bound to the immobilized antigens.
after a washing step to remove unbound sample and control material horseradish peroxidase conjugated treponemal antigens are dispensed into the wells. during a second incubation this conjugate binds specifically to antibodies resulting in the formation of enzyme-linked immune complexes.
after a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with tmb substrate and development of a blue color. the blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid.
the intensity of this color is directly proportional to the amount of treponema pallidum-specific antibody in the patient specimen. absorbance at 450 nm is read using an elisa microtiter plate reader.
this kit is intended for research use only.
not for use in diagnostic procedures.
- kit components – microtiterwells, low control, high control, calibrator, substrate solution, enzyme conjugate, stop solution, substrate solution, wash solution.
- storage – when stored at 2 °c to 8 °c, unopened reagents will retain reactivity until expiration date.
find out more about the syphilis (treponema) trep screen (20 μl sample) elisa, 96 wells and other infectious disease kits by dialing 631-589-1118, or sending an e-mail to email@example.com. you can view related products at our online store.