syphilis (treponema pallidum) igm elisa, 96 wells
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- drg international inc.
looking for syphilis (treponema pallidum) igm elisa, 96 wells from drg international inc? then approach block scientific. a leading lab apparatus distributor in new york with years of experience in the field, block scientific supplies a wide variety of consumables and accessories. we stock both new and refurbished lab equipment.
igm enzyme immunoassay kit
– solid phase elisa
the syphilis (treponema pallidum) igm elisa, 96 wells is a solid phase enzyme-linked immunosorbent assay (elisa). it provides materials for the qualitative and semiquantitative determination of igm-class antibodies to treponema pallidum in serum.
patient samples are diluted with sample diluent and additionally incubated with igg-rf-sorbent, containing hyperimmune anti-human igg-class antibody to eliminate competitive inhibition from specific igg and to remove rheumatoid factors. this pretreatment avoids false negative or false positive results.
microtiter wells as a solid phase are coated with treponema pallidum antigen.
pretreated patient specimens and ready-for-use controls are pipetted into these wells. during incubation treponema pallidum-specific antibodies of positive specimens and controls are bound to the immobilized antigens.
after a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human igm antibodies are dispensed into the wells. during a second incubation this anti-igm conjugate binds specifically to igm antibodies resulting in the formation of enzyme-linked immune complexes.
after a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with tmb substrate and development of a blue color. the blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid.
the intensity of this color is directly proportional to the amount of treponema pallidum-specific igm antibody in the patient specimen. absorbance at 450 nm is read using an elisa microtiter plate reader.
this kit is intended for research use only.
not for use in diagnostic procedures.
- kit components – microtiterwells, igg-rf-sorbent, sample diluent, neg. control, pos. control, cut-off control, substrate solution, enzyme conjugate, stop solution, wash solution
- storage – when stored at 2 °c to 8 °c unopened reagents will retain reactivity until expiration date. do not use reagents beyond this date
find out more about the syphilis (treponema pallidum) igm elisa, 96 wells and other infectious disease kits that we carry, by dialing 631-589-1118, or sending an e-mail to email@example.com. you can view related products at our online store.