syphilis (treponema pallidum) igg elisa, 96 wells
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- drg international inc.
a leading provider of laboratory equipment and supplies, block scientific offers >syphilis (treponema pallidum) igg elisa, 96 wells at competitive prices. serving the lab equipment market for many years, block scientific is a global company providing a wide range of infectious disease kits for diagnostic and research purposes.
– solid phase elisa
the drg treponema pallidum igg elisa kit is a solid phase enzyme-linked immunosorbent assay (elisa). it provides materials for the qualitative and semiquantitative determination of igg-class antibodies to treponema pallidum in serum.
microtiter wells as a solid phase are coated with treponema pallidum antigen.
diluted patient specimens and ready-for-use controls are pipetted into these wells. during incubation treponema pallidum-specific antibodies of positive specimens and controls are bound to the immobilized antigens.
after a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human igg antibodies are dispensed into the wells. during a second incubation this anti-igg conjugate binds specifically to igg antibodies resulting in the formation of enzyme-linked immune complexes.
after a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with tmb substrate and development of a blue color. the blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid.
the intensity of this color is directly proportional to the amount of treponema pallidum-specific igg antibody in the patient specimen. absorbance at 450 nm is read using an elisa microtiter plate reader.
for research use only
not for use in diagnostic procedures
- kit components – microtiterwells, calibrator, high control, low control, specimen diluent, enzyme conjugate, stop solution, substrate solution, wash solution
- storage – when stored at 2 °c to 8 °c unopened reagents will retain reactivity until expiration date
if you have any questions about the syphilis (treponema pallidum) igg elisa, 96 wells or other infectious disease kits, dial 631-589-1118 and speak to one of our customer service representatives. alternatively, you can send an email to email@example.com.