drg salivary cortisol, 96 wells
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- drg international inc.
- salivary cortisol, 96 wells
salivary cortisol, 96 wells is an enzyme immunoassay for the in vitro quantitative diagnostic measurement of active free cortisol (hydrocortisone and hydroxycorticosterone) in saliva. you can purchase this product for an amazing price from us. we are block scientific, a leading laboratory equipment supplier based in bellport, new york.
measurement for various applications
cortisol measurements with this salivary cortisol aid in the diagnosis and treatment of adrenal gland disorders.
elevated cortisol levels and lack of diurnal variation have been identified with cushing’s disease (acth hypersecretion). elevated circulating cortisol levels have also been identified in patients with adrenal tumors. low cortisol levels are found in primary adrenal insufficiency (e.g. adrenal hypoplasia, addison’s disease) and in acth deficiency. due to the normal circadian variation in cortisol levels (8), distinguishing normal from abnormally low cortisol levels can be difficult, therefore several daily collections are recommended. saliva is an excellent medium to measure steroids because it is a natural ultra-filtrate of blood, and steroids not bound by carrier proteins in the blood freely diffuse into saliva. only about 1-10% of the steroids in blood are in the unbound or free form, and it is this fraction that diffuses into target tissues of the body, and into saliva (9, 10). the majority (90-99%) of steroid hormones in the blood are bound to carrier proteins (cortisol binding globulin, sex-hormone binding globulin and albumin) and are unavailable to target tissues. the process of passive diffusion of non-bound (free) steroid hormones occurs because these small molecules are of a low molecular weight (less than 400 daltons) and are relatively nonpolar, thus enabling them to freely diffuse from blood to saliva. bound steroids are too large to diffuse freely through the salivary cells into the salivary gland lumen.
principle of the test
the drg salivary cortisol elisa kit is based on the competition principle and the microplate separation. an unknown amount of cortisol present in the sample and a fixed amount of cortisol conjugated with horse-radish peroxidase compete for the binding sites of mouse monoclonal cortisol -antiserum coated onto the wells. after one hour incubation the microplate is washed to stop the competition reaction. after addition of the substrate solution the concentration of cortisol is inversely proportional to the optical density measured.
features of the immunoassay
- assay dynamic range – between 0.537 – 80 ng/ml.
- sensitivity – the lowest detectable level of cortisol that can be distinguished from the zero standard is 0.537 ng/ml or 0.0537 μg/dl at the 95 % confidence limit.