Rubella Virus IgM ELISA*, 96 wells
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Manufacturer: DRG International Inc.
Model: Rubella Virus IgM ELISA*, 96 wells
Product Code: EIA3511
Block Scientific offers Rubella Virus IgM ELISA*, 96 wells as well as other kinds of infectious disease kits from the well known manufacturer – DRG International Inc. We are one of the prominent lab equipment dealers in the US, supplying brand name products from reliable manufacturers in the industry.
Enzyme Immunoassay Kit –
Solid Phase ELISA
The DRG Rubella Virus IgM ELISA*, 96 wells is a solid phase enzyme-linked immunosorbent assay (ELISA). It provides materials for determination of IgG-class antibodies to Rubella virus in serum.
Principle of the Test
Microtiter wells as a solid phase are coated with Rubella virus antigen.
Diluted specimens and ready-for-use controls are pipetted into these wells. During incubation Rubella virus-specific antibodies of positive specimens and controls are bound to the immobilized antigens.
After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgG antibodies are dispensed into the wells. During a second incubation this anti-IgG conjugate binds specifically to IgG antibodies resulting in the formation of enzyme-linked immune complexes.
After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid.
The intensity of this color is directly proportional to the amount of Rubella virus-specific IgG antibody in the specimen.
Absorbance at 450 nm is read using an ELISA microtiter plate reader.
THIS ASSAY IS INTENDED FOR IN VITRO USE ONLY
NOT FOR USE IN DIAGNOSTIC PROCEDURES
- Kit Components – Microtiter Wells, (Standard 1 – 3), sample diluent, high control, low control, enzyme conjugate, stop solution, substrate solution, wash solution
- Storage – When stored at 2 °C to 8 °C, unopened reagents will retain reactivity until expiration date
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