RSV (Respir. Synth.Virus) IgA ELISA, 96 wells
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Manufacturer: DRG International Inc.
Model: RSV (Respir. Synth.Virus) IgA ELISA, 96 wells
Product Code: EIA3506
Block Scientific offers RSV (Respir. Synth.Virus) IgA ELISA, 96 wells as well as other kinds of infectious disease kits from the well known manufacturer – DRG International Inc. We are one of the prominent lab equipment dealers in the US, supplying brand name products from reliable manufacturers in the industry.
IgA Elisa Kit
– Solid Phase ELISA
The DRG RSV (Respir. Synth.Virus) IgA ELISA, 96 wells is a solid phase enzyme-linked immunosorbent assay (ELISA). It provides materials for determination of IgA-class antibodies to Respiratory Syncytial Virus (RSV) in serum.
Principle of the Test
Microtiter wells as a solid phase are coated with RSV antigen.
Diluted specimens and ready-for-use controls are pipetted into these wells. During incubation RSV-specific antibodies of positive specimens and controls are bound to the immobilized antigens.
After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgA antibodies are dispensed into the wells. During a second incubation this anti-IgA conjugate binds specifically to IgA antibodies resulting in the formation of enzyme-linked immune complexes.
After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid.
The intensity of this color is directly proportional to the amount of RSV-specific IgA antibody in the specimen.
Absorbance at 450 nm is read using an ELISA microtiter plate reader.
THIS ASSAY IS INTENDED FOR RESEARCH USE ONLY
- Kit Components – microtiter wells, IgG RF Sorbent*, sample diluent, low control, high control, enzyme conjugate, calibrator, stop solution, substrate solution, wash solution
- Storage – When stored at 2 °C to 8 °C unopened reagents will retain reactivity until expiration date.
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