rsv (respir. synth.virus) iga elisa, 96 wells
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- drg international inc.
- rsv (respir. synth.virus) iga elisa, 96 wells
block scientific offers rsv (respir. synth.virus) iga elisa, 96 wells as well as other kinds of infectious disease kits from the well known manufacturer – drg international inc. we are one of the prominent lab equipment dealers in the us, supplying brand name products from reliable manufacturers in the industry.
iga elisa kit
– solid phase elisa
the drg rsv (respir. synth.virus) iga elisa, 96 wells is a solid phase enzyme-linked immunosorbent assay (elisa). it provides materials for determination of iga-class antibodies to respiratory syncytial virus (rsv) in serum.
principle of the test
microtiter wells as a solid phase are coated with rsv antigen.
diluted specimens and ready-for-use controls are pipetted into these wells. during incubation rsv-specific antibodies of positive specimens and controls are bound to the immobilized antigens.
after a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human iga antibodies are dispensed into the wells. during a second incubation this anti-iga conjugate binds specifically to iga antibodies resulting in the formation of enzyme-linked immune complexes.
after a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with tmb substrate and development of a blue color. the blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid.
the intensity of this color is directly proportional to the amount of rsv-specific iga antibody in the specimen.
absorbance at 450 nm is read using an elisa microtiter plate reader.
this assay is intended for research use only
- kit components – microtiter wells, igg rf sorbent*, sample diluent, low control, high control, enzyme conjugate, calibrator, stop solution, substrate solution, wash solution
- storage – when stored at 2 °c to 8 °c unopened reagents will retain reactivity until expiration date.
browse our online store to check out other infectious disease kits. if you have any queries about the rsv (respir. synth.virus) iga elisa, 96 wells, you can contact us by phone: 631-589-1118, or e-mail: firstname.lastname@example.org.