RSV (Respir. Synth.Virus) IgG ELISA, 96 wells
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Manufacturer: DRG International Inc.
Model: RSV (Respir. Synth.Virus) IgG ELISA, 96 wells
Product Code: EIA3507
Block Scientific offers RSV (Respir. Synth.Virus) IgG ELISA, 96 wells as well as other kinds of infectious disease kits from the well known manufacturer – DRG International Inc. We are one of the prominent lab equipment dealers in the US, supplying brand name products from reliable manufacturers in the industry.
IgG Enzyme Immunoassay Kit
– Solid Phase ELISA
The DRG RSV (Respir. Synth.Virus) IgG ELISA, 96 wells is a solid phase enzyme-linked immunosorbent assay (ELISA). It provides materials for the qualitative and semiquantitative determination of IgG-class antibodies to Respiratory Syncytial Virus (RSV) in serum.
Principle of the Test
Microtiter wells as a solid phase are coated with RSV antigen.
Diluted patient specimens and ready-for-use controls are pipetted into these wells. During incubation RSV-specific antibodies of positive specimens and controls are bound to the immobilized antigens.
After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgG antibodies are dispensed into the wells. During a second incubation this anti-IgG conjugate binds specifically to IgG antibodies resulting in the formation of enzyme-linked immune complexes.
After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid.
The intensity of this color is directly proportional to the amount of RSV-specific IgG antibody in the patient specimen.
Absorbance at 450 nm is read using an ELISA microtiter plate reader.
THIS ASSAY IS INTENDED FOR IN VITRO DIAGNOSTIC USE ONLY
- Kit Components – microtiter wells, cut-off control, sample diluent, pos. control, neg. control, enzyme conjugate, stop solution, substrate solution, wash solution
- Storage – When stored at 2 °C to 8 °C unopened reagents will retain reactivity until expiration date.
Browse our online store to check out other infectious disease kits. If you have any queries about the RSV (Respir. Synth.Virus) IgG ELISA, 96 wells, you can contact us by phone: 631-589-1118, or e-mail: firstname.lastname@example.org.