mycoplasma pneumoniae igm elisa, 96 wells
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- drg international inc.
the mycoplasma pneumoniae igm elisa, 96 wells manufactured by drg international inc. is an ideal product for various laboratory applications. block scientific is one of the prominent suppliers of laboratory equipment in the us, and we can supply this and other types of infectious disease kits.
enzyme immunoassay kit
– solid phase elisa
the drg mycoplasma pneumoniae igm elisa kit is a solid phase enzyme-linked immunosorbent assay (elisa). it provides materials for the qualitative and semiquantitative determination of igm-class antibodies to mycoplasma pneumoniae in serum.
patient samples are diluted with sample diluent and additionally incubated with igg-rf-sorbent, containing hyperimmune anti-human igg-class antibody to eliminate competitive inhibition from specific igg and to remove rheumatoid factors. this pretreatment avoids false negative or false positive results.
microtiter wells as a solid phase are coated with mycoplasma pneumoniae antigen.
pretreated patient specimens and ready-for-use controls are pipetted into these wells. during incubation mycoplasma pneumoniae-specific antibodies of positive specimens and controls are bound to the immobilized antigens.
after a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human igm antibodies are dispensed into the wells. during a second incubation this anti-igm conjugate binds specifically to igm antibodies resulting in the formation of enzyme-linked immune complexes.
after a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with tmb substrate and development of a blue color. the blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid.
the intensity of this color is directly proportional to the amount of mycoplasma pneumoniae-specific igm antibody in the patient specimen. absorbance at 450 nm is read using an elisa microtiter plate reader.
intended for in vitro use only
in the united states, this kit is intended for research use only
- kit contents – microtiterwells, igg-rf-sorbent, sample diluent, low control, high control, calibrator, substrate solution, enzyme conjugate, wash solution, stop solution
- storage – when stored at 2 °c to 8 °c unopened reagents will retain reactivity until expiration date.
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for more information on the mycoplasma pneumoniae igm elisa, 96 wells and other infectious disease kits we carry, please visit us at our online store. we are also available on phone at 631-589-1118 or by e-mail at email@example.com.