Mycoplasma pneumoniae IgG ELISA, 96 wells

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Mycoplasma pneumoniae IgG ELISA, 96 wells

Manufacturer: DRG International Inc.
Model: Mycoplasma pneumoniae IgG ELISA, 96 wells
Product Code: EIA3499

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Block Scientific offers Mycoplasma pneumoniae IgG ELISA, 96 wells as well as other kinds of infectious disease kits from the well known manufacturer – DRG International Inc. We are one of the prominent lab equipment dealers in the US, supplying brand name products from reliable manufacturers in the industry.

IgG Elisa Kit

– Solid Phase ELISA

The DRG Mycoplasma pneumoniae IgG ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA. It provides materials for the the qualitative and semiquantitative determination of IgG-class antibodies to Mycoplasma pneumoniae in serum.

PRINCIPLE OF THE TEST

The DRG Mycoplasma pneumoniae IgG ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA).

Microtiter wells as a solid phase are coated with Mycoplasma pneumoniae antigen.

Diluted patient specimens and ready-for-use controls are pipetted into these wells. During incubation Mycoplasma pneumoniae-specific antibodies of positive specimens and controls are bound to the immobilized antigens.

After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgG antibodies are dispensed into the wells. During a second incubation this anti-IgG conjugate binds specificly to IgG antibodies resulting in the formation of enzyme-linked immune complexes.

After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid.

The intensity of this color is directly proportional to the amount of Mycoplasma pneumoniae-specific IgG antibody in the patient specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.

Features

FOR IN VITRO DIAGNOSTIC USE ONLY

  • Kit Components – microtiterwells, sample diluent, negative control, positive control, enzyme conjugate, cut-off control, stop solution, substrate solution, wash solution
  • Storage – When stored at 2 °C to 8 °C unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date

Browse our online store to check out other infectious disease kits. If you have any queries about the Mycoplasma pneumoniae IgG ELISA, 96 wells, you can contact us by phone: 631-589-1118, or e-mail: info@blockscientific.com.