mycoplasma hominis igg elisa, 96 wells
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- drg international inc.
- mycoplasma hominis igg elisa, 96 wells
block scientific offers mycoplasma hominis igg elisa, 96 wells as well as other kinds of infectious disease kits from the well known manufacturer – drg international inc. we are one of the prominent lab equipment dealers in the us, supplying brand name products from reliable manufacturers in the industry.
igg elisa kit
– solid phase elisa
the drg mycoplasma hominis igg elisa kit is a solid phase enzyme-linked immunosorbent assay (elisa). it provides materials for the qualitative and semiquantitative determination of iga-class antibodies to mycoplasma hominis in serum.
principle of the test
patient samples are diluted with sample diluent and additionally incubated with igg-rf-sorbent, containing hyperimmune anti-human igg-class antibody to eliminate competitive inhibition from specific igg and to remove rheumatoid factors. this pretreatment avoids false negative or false positive results.
microtiter wells as a solid phase are coated with mycoplasma hominis antigen.
diluted patient specimens and ready-for-use controls are pipetted into these wells. during incubation mycoplasma hominis-specific antibodies of positive specimens and controls are bound to the immobilized antigens.
after a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human iga antibodies are dispensed into the wells. during a second incubation this anti-iga conjugate binds specifically to iga antibodies resulting in the formation of enzyme-linked immune complexes.
after a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with tmb substrate and development of a blue color. the blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid.
the intensity of this color is directly proportional to the amount of mycoplasma hominis-specific iga antibody in the patient specimen. absorbance at 450 nm is read using an elisa microtiter plate reader.
- kit components – microtiterwells, sample diluent, negative control, positive control, enzyme conjugate, cut-off control, stop solution, substrate solution, wash solution
- storage – when stored at 2 °c to 8 °c unopened reagents will retain reactivity until expiration date.
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