Measles Virus IgM ELISA, 96 wells

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Measles Virus IgM ELISA, 96 wells

Manufacturer: DRG International Inc.
Model: Measles Virus IgM ELISA, 96 wells
Product Code: EIA3496

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Block Scientific is the laboratory equipment supplier you can depend on for all kinds of infectious disease kits. So you have come to the right place to purchase the Measles Virus IgM ELISA, 96 wells. Based in Bellport, New York, we have more than 35 years of experience in dealing in a comprehensive range of lab equipment for both domestic and international clients.

DRG Measles Virus IgM ELISA Kit

This Enzyme Immunoassay Kit provides materials for the qualitative and semi-quantitative determination of IgM-class antibodies to Measles Virus in serum. This assay is intended for in vitro use only. In the United States, this kit is intended for Research Use Only.

Test Principle

The DRG Measles Virus IgM ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA). Patient samples are diluted with Sample Diluent and additionally incubated with IgG-RF-Sorbent, containing hyper-immune anti-human IgG-class antibody to eliminate competitive inhibition from specific IgG and to remove rheumatoid factors. This pretreatment avoids false negative or false positive results.

Microtiter wells as a solid phase are coated with inactivated Measles Virus antigen (strain Edmonston). Diluted patient specimens and ready-for-use controls are pipetted into these wells. During incubation MeaslesVirus-specific antibodies of positive specimens and controls are bound to the immobilized antigens. After a washing step to remove unbound sample and control material horseradish peroxidase conjugated antihuman IgM antibodies are dispensed into the wells. During a second incubation this anti-IgM conjugate binds specifically to IgM antibodies resulting in the formation of enzyme-linked immune complexes. After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directly proportional to the amount of Measles Virus-specific IgM.

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