Measles Virus IgG ELISA, 96 wells
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Manufacturer: DRG International Inc.
Model: Measles Virus IgG ELISA, 96 wells
Product Code: EIA3495
Block Scientific is the laboratory equipment supplier you can depend on for all kinds of infectious disease kits. So you have come to the right place to purchase the Measles Virus IgG ELISA, 96 wells. Based in Bellport, New York, we have more than 35 years of experience in dealing in a comprehensive range of lab equipment for both domestic and international clients.
– Useful Assay
This assay is a useful product for use by appropriately trained and qualified personnel for the detection of antibodies to P.falciparum, P.vivax, P.ovale and P.malariae in human serum and plasma.
The DRG Measles Virus IgG ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA).
Microtiter wells as a solid phase are coated with inactivated Measles Virus antigen (strain Edmonston).
Diluted patient specimens and ready-for-use controls are pipetted into these wells. During incubation Measles Virusspecific antibodies of positive specimens and controls are bound to the immobilized antigens.
After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgG antibodies are dispensed into the wells. During a second incubation this anti-IgG conjugate binds specifically to IgG antibodies resulting in the formation of enzyme-linked immune complexes.
After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid.
The intensity of this color is directly proportional to the amount of Measles Virus-specific IgG antibody in the patient specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.
FOR IN VITRO DIAGNOSTIC USE ONLY
- Kit Contents – Negative control, microtiterwells, positive control, sample diluent, cut-off control, substrate solution, enzyme conjugate, wash solution, stop solution
- Storage – When stored at 2 °C to 8 °C unopened reagents will retain reactivity until expiration date.
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