Malaria Ab ELISA, 96 Wells, Min 10 kits per order
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Manufacturer: DRG International Inc.
Model: Malaria Ab ELISA, 96 Wells, Min 10 kits per order
Product Code: EIA5048
Block Scientific is the laboratory equipment supplier you can depend on for all kinds of infectious disease kits. So you have come to the right place to purchase the Malaria Ab ELISA, 96 Wells, Min 10 kits per order. Based in Bellport, New York, we have more than 35 years of experience in dealing in a comprehensive range of lab equipment for both domestic and international clients.
– Useful Assay
This assay is a 3rd Generation Enzyme Immunoassay (ELISA) for the determination of antibodies to Plasmodium species in human sera and plasma.
Recombinant proteins representing immunodominant epitopes of Plasmodium species, are coated onto wells of a microplate.
Recombinant proteins have been carefully selected to ensure the screening of all antibodies to P. species. Serum or plasma samples are added to these wells and, if antibodies specific to P. species (IgG, IgM or IgA) are present in the sample, they will form stable complexes with the recombinant antigens in the well.
Antigen-antibody complexes are then identified through the successive addition of: (1) same biotinylated recombinant proteins specific to P. species and; (2) horseradish peroxidase HRP Streptavidin conjugate.
The hydrolytic activity of horseradish peroxidase allows for the quantification of these antibody-antigen complexes.
Peroxidase substrate solution is then added.
During incubation, a blue colour will develop in proportion to the amount of anti P. species antibodies bound to the well, thus establishing their presence or absence in the sample. Wells containing samples negative for anti-P. species antibody remain colourless.
A stop solution is added to each well and the resulting yellow colour is read on a microplate reader at 450 nm.
THIS KIT IS INTENDED FOR RESEARCH USE ONLY
NOT FOR USE IN DIAGNOSTIC PROCEDURES
- Kit Contents – Negative control, microplate, positive control, calibrator, Conjugate # 1, Wash buffer concentrate 20X, Conjugate 1 Diluent (CONJ 1 DIL), Conjugate # 2 (CONJ 2), Stop Solution (Sulphuric Acid) (H2SO4 0.3M), Chromogen/Substrate (SUBS TMB), Sample Diluent : (DILSPE), package insert, plate sealing foils
- Storage – A study conducted on an opened kit has not pointed out any relevant loss of activity up to 2 months.
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