leptin (sandwich eia), 96 wells
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- drg international inc.
- leptin (sandwich eia), 96 wells
block scientific offers leptin (sandwich eia), 96 wells – an enzyme immunoassay for the measurement of leptin in serum and plasma, for an affordable price. we are one of the leading laboratory equipment supliers in the united states offering brand new as well as refurbished lab equipment for various medical, scientific, research laboratories and healthcare facilities.
drg leptin elisa
– based on sandwich principle
the drg leptin elisa kit is a solid phase enzyme-linked immunosorbent assay (elisa) based on the sandwich principle.
the microtiter wells are coated with a monoclonal antibody directed towards a unique antigenic site on a leptin molecule. an aliquot of patient sample containing endogenous leptin is incubated in the coated well with a specific rabbit anti leptin antibody. a sandwich complex is formed. after incubation the unbound material is washed off and an anti rabbit peroxidase conjugate is added for detection of the bound leptin.
having added the substrate solution, the intensity of colour developed is proportional to the concentration of leptin in the patient sample.
- reagents provided – microtiterwells, control (low & high), standard (standard 0-5), antiserum, assay buffer, substrate solution, enzyme complex, wash solution, stop solution
- storage – when stored at 2-8°c unopened reagents will retain reactivity until expiration date.
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