influenza b igm elisa, 96 wells
to add this product to your interest list, sign up or login.
- drg international inc.
- influenza b igm elisa, 96 wells
block scientific is your ideal provider if you are looking to buy the influenza b igm elisa, 96 wells for a competitive price. ours is a leading firm in the lab equipment industry, known for distributing laboratory apparatuses belonging to various brands.
influenza b test
– solid phase elisa
this product is a solid phase enzyme-linked immunosorbent assay (elisa). it provides materials for the qualitative and semiquantitative determination of igg-class antibodies to influenza virus b in serum.
patient samples are diluted with sample diluent and additionally incubated with igg-rf-sorbent, containing hyper-immune anti-human igg-class antibody to eliminate competitive inhibition from specific igg and to remove rheumatoid factors. this pretreatment avoids false negative or false positive results.
microtiter wells as a solid phase are coated with influenza virus b antigen.
diluted patient specimens and ready-for-use controls are pipetted into these wells. during incubation influenza virus b -specific antibodies of positive specimens and controls are bound to the immobilized antigens.
after a washing step to remove unbound sample and control material horseradish peroxidase conjugated antihuman igm antibodies are dispensed into the wells. during a second incubation this anti-igm conjugate binds specifically to igm antibodies resulting in the formation of enzyme-linked immune complexes.
after a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with tmb substrate and development of a blue color. the blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid.
the intensity of this color is directly proportional to the amount of influenza virus b-specific igm antibody in the patient specimen. absorbance at 450 nm is read using an elisa microtiter plate reader.
intended for in vitro use only
in the united states, this kit is intended for research use only.
- contents of the kit – microtiterwells, positive control, negative control, sample diluent, cut-off control, substrate solution, igg-rf-sorbent *, enzyme conjugate, wash solution, stop solution
- storage – when stored at 2 °c to 8 °c unopened reagents will retain reactivity until expiration date.
for more information regarding the influenza b igm elisa, 96 wells, dial 631-589-1118, or send an e-mail to email@example.com. to know about other models of infectious disease kits we supply, visit us at our online store.