Influenza A IgM ELISA, 96 Wells

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Influenza A IgM ELISA, 96 Wells

Manufacturer: DRG International Inc.
Model: Influenza A IgM ELISA, 96 wells
Product Code: EIA4240

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Block Scientific is your ideal provider if you are looking to buy the Influenza A IgM ELISA, 96 wells for a competitive price. Ours is a leading firm in the lab equipment industry, known for distributing laboratory apparatuses belonging to various brands.

Elisa Kit

– Solid Phase ELISA

This product is a solid phase enzyme-linked immunosorbent assay (ELISA). It provides materials for the qualitative and semiquantitative determination of IgM-class antibodies to Influenza-Virus A in serum.

Test Principle

Patient samples are diluted with Sample Diluent and additionally incubated with IgG-RF-Sorbent, containing hyper-immune anti-human IgG-class antibody to eliminate competitive inhibition from specific IgG and to remove rheumatoid factors. This pretreatment avoids false negative or false positive results.

Microtiter wells as a solid phase are coated with Influenza-Virus A antigen.

Diluted patient specimens and ready-for-use controls are pipetted into these wells. During incubation Influenza-Virus A-specific antibodies of positive specimens and controls are bound to the immobilized antigens.

After a washing step to remove unbound sample and control material horseradish peroxidase conjugated antihuman IgM antibodies are dispensed into the wells. During a second incubation this anti-IgM conjugate binds specifically to IgM antibodies resulting in the formation of enzyme-linked immune complexes.

After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid.

The intensity of this color is directly proportional to the amount of Influenza-Virus A-specific IgM antibody in the patient specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.




  • Contents of the Kit – microtiterwells, high control, low control, sample diluent, calibrator, substrate solution, IgG-RF-Sorbent *, enzyme conjugate, wash solution, stop solution
  • Storage – When stored at 2 °C to 8 °C unopened reagents will retain reactivity until expiration date.

Contact Us

For more information regarding the Influenza A IgM ELISA, 96 wells, dial 631-589-1118, or send an e-mail to To know about other models of infectious disease kits we supply, visit us at our online store.