influenza a igg elisa, 96 wells
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- drg international inc.
- influenza a igg elisa, 96 wells
block scientific is your ideal provider if you are looking to purchase influenza a igg elisa, 96 wells for a competitive price. ours is a leading firm in the industry, known for distributing lab equipment belonging to various well known brands.
enzyme immunoassay kit
– solid-phase elisa
the drg influenza a igg elisa kit is a solid phase enzyme-linked immunosorbent assay (elisa). it provides materials for the qualitative and semiquantitative determination of iga-class antibodies to influenza virus a in serum.
microtiter wells as a solid phase are coated with influenza virus a antigen.
diluted patient specimens and ready-for-use controls are pipetted into these wells. during incubation influenza virus a-specific antibodies of positive specimens and controls are bound to the immobilized antigens.
after a washing step to remove unbound sample and control material horseradish peroxidase conjugated antihuman igg antibodies are dispensed into the wells. during a second incubation this anti-igg conjugate binds specificly to igg antibodies resulting in the formation of enzyme-linked immune complexes.
after a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with tmb substrate and development of a blue color. the blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid.
the intensity of this color is directly proportional to the amount of influenza virus a-specific igg antibody in the patient specimen. absorbance at 450 nm is read using an elisa microtiter plate reader.
it is supplied for research purpose only. it is not for use in the diagnosis or for the follow-up of patients administered with the vaccines containing hpv antigens.
- kit contents – microtiterwells, igg-rf-sorbent, sample diluent, low control, high control, calibrator, substrate solution, enzyme conjugate, wash solution, stop solution
- storage – when stored at 2 °c to 8 °c unopened reagents will retain reactivity until expiration date.
for more information regarding influenza a igg elisa, 96 wells and other types of infectious disease kits we supply, dial 631-589-1118 or send an e-mail to email@example.com. to check out other products we deal in, please visit our online store.