herpes simplex-2 igm elisa, 96 wells
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- drg international inc.
- herpes simplex-2 igm elisa, 96 wells
looking for herpes simplex-2 igm elisa, 96 wells from drg international inc? then approach block scientific. a leading lab apparatus distributor in new york with years of experience in the field, block scientific supplies a wide variety of consumables and accessories. we stock both new and refurbished lab equipment.
herpes simplex 2 assay
– solid phase elisa
the herpes simplex-2 igm elisa, 96 wells is a solid phase enzyme-linked immunosorbent assay (elisa). it provides materials for the qualitative and semiquantitative determination of igm-class antibodies to hsv-2 in serum.
patient samples are diluted with sample diluent and additionally incubated with igg-rf-sorbent, containing hyper-immune anti-human igg-class antibody to eliminate competitive inhibition from specific igg and to remove rheumatoid factors. this pretreatment avoids false negative or false positive results.
microtiter wells as a solid phase are coated with hsv-2 antigen.
diluted patient specimens and ready-for-use controls are pipetted into these wells. during incubation hsv-2-specific antibodies of positive specimens and controls are bound to the immobilized antigens.
after a washing step to remove unbound sample and control material horseradish peroxidase conjugated antihuman igm antibodies are dispensed into the wells. during a second incubation this anti-igm conjugate binds specificly to igm antibodies resulting in the formation of enzyme-linked immune complexes.
after a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with tmb substrate and development of a blue color. the blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid.
the intensity of this color is directly proportional to the amount of hsv-2-specific igm antibody in the patient specimen. absorbance at 450 nm is read using an elisa microtiter plate reader.
- kit components – microtiterwells, sample diluent, cut-off control, pos. control, neg. control, igg-rf-sorbent, enzyme conjugate, stop solution, substrate solution, wash solution
- storage – when stored at 2 °c to 8 °c, unopened reagents will retain reactivity until expiration date.
find out more about the herpes simplex-2 igm elisa, 96 wells and other infectious disease kits by dialing 631-589-1118, or sending an e-mail to firstname.lastname@example.org. you can view related products at our online store.