Herpes Simplex-2 IgM ELISA, 96 wells

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Herpes Simplex-2 IgM ELISA, 96 wells

Manufacturer: DRG International Inc.
Model: Herpes Simplex-2 IgM ELISA, 96 wells
Product Code: EIA3488

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Looking for Herpes Simplex-2 IgM ELISA, 96 wells from DRG International Inc? Then approach Block Scientific. A leading lab apparatus distributor in New York with years of experience in the field, Block Scientific supplies a wide variety of consumables and accessories. We stock both new and refurbished lab equipment.

Herpes Simplex 2 Assay

– Solid Phase ELISA

The Herpes Simplex-2 IgM ELISA, 96 wells is a solid phase enzyme-linked immunosorbent assay (ELISA). It provides materials for the qualitative and semiquantitative determination of IgM-class antibodies to HSV-2 in serum.

Test Principle

Patient samples are diluted with Sample Diluent and additionally incubated with IgG-RF-Sorbent, containing hyper-immune anti-human IgG-class antibody to eliminate competitive inhibition from specific IgG and to remove rheumatoid factors. This pretreatment avoids false negative or false positive results.

Microtiter wells as a solid phase are coated with HSV-2 antigen.

Diluted patient specimens and ready-for-use controls are pipetted into these wells. During incubation HSV-2-specific antibodies of positive specimens and controls are bound to the immobilized antigens.

After a washing step to remove unbound sample and control material horseradish peroxidase conjugated antihuman IgM antibodies are dispensed into the wells. During a second incubation this anti-IgM conjugate binds specificly to IgM antibodies resulting in the formation of enzyme-linked immune complexes.

After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid.

The intensity of this color is directly proportional to the amount of HSV-2-specific IgM antibody in the patient specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.

Features

  • Kit Components – microtiterwells, sample diluent, cut-off control, pos. control, neg. control, IgG-RF-Sorbent, enzyme conjugate, stop solution, substrate solution, wash solution
  • Storage – When stored at 2 °C to 8 °C, unopened reagents will retain reactivity until expiration date.

Find out more about the Herpes Simplex-2 IgM ELISA, 96 wells and other infectious disease kits by dialing 631-589-1118, or sending an e-mail to info@blockscientific.com. You can view related products at our online store.