buy now

herpes simplex-2 igm elisa, 96 wells

to add this product to your interest list, sign up or login.

herpes simplex-2 igm elisa, 96 wells
  1. drg international inc.
  2. herpes simplex-2 igm elisa, 96 wells
  3. eia3488

looking for herpes simplex-2 igm elisa, 96 wells from drg international inc? then approach block scientific. a leading lab apparatus distributor in new york with years of experience in the field, block scientific supplies a wide variety of consumables and accessories. we stock both new and refurbished lab equipment.

herpes simplex 2 assay

– solid phase elisa

the herpes simplex-2 igm elisa, 96 wells is a solid phase enzyme-linked immunosorbent assay (elisa). it provides materials for the qualitative and semiquantitative determination of igm-class antibodies to hsv-2 in serum.

test principle

patient samples are diluted with sample diluent and additionally incubated with igg-rf-sorbent, containing hyper-immune anti-human igg-class antibody to eliminate competitive inhibition from specific igg and to remove rheumatoid factors. this pretreatment avoids false negative or false positive results.

microtiter wells as a solid phase are coated with hsv-2 antigen.

diluted patient specimens and ready-for-use controls are pipetted into these wells. during incubation hsv-2-specific antibodies of positive specimens and controls are bound to the immobilized antigens.

after a washing step to remove unbound sample and control material horseradish peroxidase conjugated antihuman igm antibodies are dispensed into the wells. during a second incubation this anti-igm conjugate binds specificly to igm antibodies resulting in the formation of enzyme-linked immune complexes.

after a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with tmb substrate and development of a blue color. the blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid.

the intensity of this color is directly proportional to the amount of hsv-2-specific igm antibody in the patient specimen. absorbance at 450 nm is read using an elisa microtiter plate reader.


  • kit components – microtiterwells, sample diluent, cut-off control, pos. control, neg. control, igg-rf-sorbent, enzyme conjugate, stop solution, substrate solution, wash solution
  • storage – when stored at 2 °c to 8 °c, unopened reagents will retain reactivity until expiration date.

find out more about the herpes simplex-2 igm elisa, 96 wells and other infectious disease kits by dialing 631-589-1118, or sending an e-mail to you can view related products at our online store.