Herpes Simplex 1+2 IgM ELISA, 96 Wells

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Herpes Simplex 1+2 IgM ELISA, 96 Wells

Manufacturer: DRG International Inc.
Model: Herpes Simplex 1+2 IgM ELISA, 96 wells
Product Code: EIA3490

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Looking for Herpes Simplex 1+2 IgM ELISA, 96 wells from DRG International Inc? Then approach Block Scientific. A leading lab apparatus distributor in New York with years of experience in the field, Block Scientific supplies a wide variety of consumables and accessories. We stock both new and refurbished lab equipment.

Enzyme Immunoassay

– Solid Phase ELISA

The Herpes Simplex 1+2 IgM ELISA, 96 wells is a solid phase enzyme-linked immunosorbent assay (ELISA). It is useful for determination of IgM-class antibodies to Herpes Simplex Virus Type 1 + 2 in human serum.

Test Principle

Samples are diluted with Sample Diluent and additionally incubated with IgG-RF-Sorbent, containing hyper-immune anti-human IgG-class antibody to eliminate competitive inhibition from specific IgG and to remove rheumatoid factors.

This pretreatment avoids false negative or false positive results.

Microtiter wells as a solid phase are coated with Herpes Simplex Virus Type 1 + 2 antigen.

Pretreated specimens and ready-for-use controls are pipetted into these wells. During incubation Herpes Simplex Virus Type 1 + 2-specific antibodies of positive specimens and controls are bound to the immobilized antigens.

After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgM antibodies are dispensed into the wells. During a second incubation this anti-IgM conjugate binds specifically to IgM antibodies resulting in the formation of enzyme-linked immune complexes.

After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid.

The intensity of this color is directly proportional to the amount of Herpes Simplex Virus Type 1 + 2-specific IgM antibody in the specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.

Features

FOR RESEARCH USE ONLY

  • Kit Components – microtiterwells, sample diluent, cut-off control, pos. control, neg. control, IgG-RF-Sorbent, enzyme conjugate, stop solution, substrate solution, wash solution
  • Storage – When stored at 2 °C to 8 °C, unopened reagents will retain reactivity until expiration date.

Find out more about the Herpes Simplex 1+2 IgM ELISA, 96 wells and other infectious disease kits by dialing 631-589-1118, or sending an e-mail to info@blockscientific.com. You can view related products at our online.