herpes simplex 1+2 igg elisa, 96 wells
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- drg international inc.
- herpes simplex 1+2 igg elisa, 96 wells
looking for herpes simplex 1+2 igg elisa, 96 wells from drg international inc? then approach block scientific. a leading lab apparatus distributor in new york with years of experience in the field, block scientific supplies a wide variety of consumables and accessories. we stock both new and refurbished lab equipment.
– solid phase elisa
the herpes simplex 1+2 igg elisa, 96 wells is a solid phase enzyme-linked immunosorbent assay (elisa). it provides materials for for the qualitative and semiquantitative determination of igg-class antibodies to herpes simplex virus type 1+ 2 in human serum.
microtiter wells as a solid phase are coated with herpes simplex virus type 1 antigen.
diluted patient specimens and ready-for-use controls are pipetted into these wells. during incubation herpes simplex virus type 1+2-specific antibodies of positive specimens and controls are bound to the immobilized antigens.
after a washing step to remove unbound sample and control material horseradish peroxidase conjugated antihuman igg antibodies are dispensed into the wells. during a second incubation this anti-igg conjugate binds specifically to igg antibodies resulting in the formation of enzyme-linked immune complexes.
after a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with tmb substrate and development of a blue color. the blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid.
the intensity of this color is directly proportional to the amount of herpes simplex virus type 1+2-specific igg antibody in the patient specimen. absorbance at 450 nm is read using an elisa microtiter plate reader.
for in vitro use only.
in the united states, this kit is intended for research use only.
- kit components – microtiterwells, sample diluent, cut-off control, pos. control, neg. control, enzyme conjugate, stop solution, substrate solution, wash solution
- storage – when stored at 2 °c to 8 °c, unopened reagents will retain reactivity until expiration date.
find out more about the herpes simplex 1+2 igg elisa, 96 wells and other infectious disease kits by dialing 631-589-1118, or sending an e-mail to firstname.lastname@example.org. you can view related products at our online store.