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herpes simplex-1 igm elisa, 96 wells

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herpes simplex-1 igm elisa, 96 well
  1. drg international inc.
  2. herpes simplex-1 igm elisa, 96 wells
  3. eia3486

looking for herpes simplex-1 igm elisa, 96 wells from drg international inc? then approach block scientific. a leading lab apparatus distributor in new york with years of experience in the field, block scientific supplies a wide variety of consumables and accessories. we stock both new and refurbished lab equipment.

herpes simplex assay

– solid phase elisa

the herpes simplex-1 igm elisa, 96 wells is a solid phase enzyme-linked immunosorbent assay (elisa). it provides materials for the qualitative and semiquantitative determination of igm-class antibodies to hsv-1 in serum. this assay is intended for in vitro use only.

test principle

patient samples are diluted with sample diluent and additionally incubated with igg-rf-sorbent, containing hyperimmune anti-human igg-class antibody to eliminate competitive inhibition from specific igg and to remove rheumatoid factors. this pretreatment avoids false negative or false positive results.

microtiter wells as a solid phase are coated with hsv-1 antigen.

diluted patient specimens and ready-for-use controls are pipetted into these wells. during incubation hsv-1-specific antibodies of positive specimens and controls are bound to the immobilized antigens.

after a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human igm antibodies are dispensed into the wells. during a second incubation this anti-igm conjugate binds specifically to igm antibodies resulting in the formation of enzyme-linked immune complexes.

after a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with tmb substrate and development of a blue color. the blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid.

the intensity of this color is directly proportional to the amount of hsv-1-specific igm antibody in the patient specimen.

absorbance at 450 nm is read using an elisa microtiter plate reader.


  • kit components – microtiterwells, igg-rf-sorbent, sample diluent, low control, high control, enzyme conjugate, calibrator, stop solution, substrate solution, wash solution
  • storage – when stored at 2 °c to 8 °c, unopened reagents will retain reactivity until expiration date.

find out more about the herpes simplex-1 igm elisa, 96 wells and other infectious disease kits by dialing 631-589-1118, or sending an e-mail to you can view related products at our online store.