Herpes Simplex-1 IgG ELISA, 96 Wells

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Herpes Simplex-1 IgG ELISA, 96 Wells

Manufacturer: DRG International Inc.
Model: Herpes Simplex-1 IgG ELISA, 96 wells
Product Code: EIA3485

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Block Scientific is a reputable distributor in the United States, offering a fine line of lab equipment for sale including chemistry analyzers, hematology analyzers, centrifuges, histology equipment, reagents and consumables. Based in Bellport, New York, we offer the Herpes Simplex-1 IgG ELISA, 96 wells and other infectious disease kits at competitive prices.

Herpes Simplex Assay

– Solid Phase ELISA

The DRG Herpes Simplex Virus Type 1 IgG ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA). It provides materials for the qualitative and semiquantitative determination of IgG-class antibodies to Herpes Simplex Virus Type 1 in serum.

Test Principle

Microtiter wells as a solid phase are coated with Herpes Simplex Virus Type 1 antigen.

Diluted patient specimens and ready-for-use controls are pipetted into these wells. During incubation Herpes Simplex Virus Type 1-specific antibodies of positive specimens and controls are bound to the immobilized antigens.

After a washing step to remove unbound sample and control material horseradish peroxidase conjugated antihuman IgG antibodies are dispensed into the wells. During a second incubation this anti-IgG conjugate binds specifically to IgG antibodies resulting in the formation of enzyme-linked immune complexes.

After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid.

The intensity of this color is directly proportional to the amount of Herpes Simplex Virus Type 1-specific IgG antibody in the patient specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.

Features

INTENDED FOR IN VITRO USE ONLY. FOR RESEARCH USE ONLY.

  • Test Kit Contents – microtiterwells, cut-off control, negative control, positive control, substrate solution, enzyme conjugate, wash solution, stop solution, sample diluent
  • Storage – When stored at 2 °C to 8 °C, unopened reagents will retain reactivity until expiration date.

For more information on the Herpes Simplex-1 IgG ELISA, 96 wells and other infectious disease kits, please make a call to 631-589-1118, or send an e-mail to info@blockscientific.com. To view other products we carry, please browse through our online store.