helicobacter pylori igg (cag a) elisa, 96 wells
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- drg international inc.
block scientific is a reputable distributor in the united states, offering a fine line of lab equipment for sale including chemistry analyzers, hematology analyzers, centrifuges, histology equipment, reagents and consumables. based in bellport, new york, we offer the helicobacter pylori igg (cag a) elisa, 96 wells and other infectious disease kits at competitive prices.
helicobacter pylori assay
– solid phase elisa
the drg helicobacter pylori igg elisa kit is a solid phase enzyme-linked immunosorbent assay (elisa). it provides materials for the quantitative and qualitative determination of igg-class antibodies to helicobacter pylori in serum.
microtiter wells as a solid phase are coated with recombinant helicobacter pylori cag a antigen.
diluted patient specimens and ready-for-use controls are pipetted into these wells. during incubation helicobacter pylori-specific antibodies of positive specimens and controls are bound to the immobilized antigens.
after a washing step to remove unbound sample and control material horseradish peroxidase conjugated antihuman igg antibodies are dispensed into the wells. during a second incubation this anti-igg conjugate binds specifically to igg antibodies resulting in the formation of enzyme-linked immune complexes.
after a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with tmb substrate and development of a blue color. the blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid.
the intensity of this color is directly proportional to the amount of helicobacter pylori-specific igg antibody in the patient specimen. absorbance at 450 nm is read using an elisa microtiter plate reader.
intended for in vitro use only
- kit components – microtiterwells, sample diluent, neg. control, pos. control, standard (standard 1 – 3)*, substrate solution, enzyme conjugate, wash solution, stop solution
- storage – when stored at 2 °c to 8 °c unopened reagents will retain reactivity until expiration date.
for more information on the helicobacter pylori igg (cag a) elisa, 96 wells and other infectious disease kits please make a call to 631-589-1118, or send an e-mail to firstname.lastname@example.org. to view other products we carry, please browse through our online store.