Helicobacter Pylori IgA ELISA, 96 Wells
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Manufacturer: DRG International Inc.
Model: Helicobacter Pylori IgA ELISA, 96 wells
Product Code: EIA3483
Block Scientific is a reputable distributor in the United States, offering a fine line of lab equipment for sale including chemistry analyzers, hematology analyzers, centrifuges, histology equipment, reagents and consumables. Based in Bellport, New York, we offer the Helicobacter Pylori IgA ELISA, 96 wells and other infectious disease kits at competitive prices.
Helicobacter Pylori Assay
– Solid Phase ELISA
The DRG Helicobacter pylori IgA ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA). It provides materials for determination of IgA-class antibodies to Helicobacter pylori in serum.
Samples are diluted with Sample Diluent and additionally incubated with IgG-RF-Sorbent, containing hyper-immune antihuman IgG-class antibody to eliminate competitive inhibition from specific IgG and to remove rheumatoid factors. This pretreatment avoids false negative or false positive results.
Microtiter wells as a solid phase are coated with recombinant Helicobacter pylori (CagA) antigen.
Diluted specimens and ready-for-use controls are pipetted into these wells. During incubation Helicobacter pylori-specific antibodies of positive specimens and controls are bound to the immobilized antigens.
After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgA antibodies are dispensed into the wells. During a second incubation this anti-IgA conjugate binds specifically to IgA antibodies resulting in the formation of enzyme-linked immune complexes.
After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid.
The intensity of this color is directly proportional to the amount of Helicobacter pylori-specific IgA antibody in the specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.
INTENDED FOR RESEARCH USE ONLY, NOT FOR DIAGNOSTIC PROCEDURES
- Kit components – microtiterwells, IgG-RF-Sorbent*, sample diluent, low control (standard 0)*, high control*, standard (standard 1 – 3)*, enzyme conjugate, stop solution, substrate solution, wash solution
- Storage – When stored at 2 °C to 8 °C unopened reagents will retain reactivity until expiration date.
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