hdv ab elisa, 96 wells, min 10 kits per order
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- drg international inc.
a leading provider of laboratory equipment and supplies, block scientific offers hdv ab elisa*, 96 wells, min 10 kits per order at competitive prices. serving the lab equipment market for many years, block scientific is a global company providing a wide range of infectious disease kits for diagnostic and research purposes.
hdv ab assay
– competitive elisa
this product is a competitive enzyme immunoassay (elisa) for the determination of antibodies to hepatitis delta virus or hdv in human plasma and sera with a “two-steps” methodology.
anti-hdv antibodies, if present in the sample, compete with a virus-specific polyclonal igg, labeled with peroxidase (hrp), for a fixed amount of rec-hdv coated on the microplate.
the test is carried out with a two steps incubation competitive system. first the sample is added to the plate and specific anti hdv antibodies bind to the adsorbed antigen. after washing, an enzyme conjugated polyclonal antibody to hdv is added and binds to the free portion of the antigen coated.
after washing a chromogen/substrate mixture is dispensed.
the concentration of the bound enzyme on the solid phase becomes inversely proportional to the amount of anti-hdv antibodies in the sample and its activity is detected by the added chromogen/substrate.
the concentration of hdv-specific antibodies in the sample is determined by means of a cut-off value that allows for the semi quantitative detection of anti-hdv.
for research use only
- kit components – microplate, calibrator, positive control, negative control, enzyme conjugate, wash buffer concentrate, sulphuric acid, chromogen/substrate
- a study conducted on an opened kit has not pointed out any relevant loss of activity up to 6 re-uses of the device and up to 3 months.
if you have any questions about the hdv ab elisa*, 96 wells, min 10 kits per order or other infectious disease kits, dial 631-589-1118 and speak to one of our customer service representatives. alternatively, you can send an email to firstname.lastname@example.org.