hbc ab screening, 96 wells
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- drg international inc.
- hbc ab screening, 96 wells
if you are in search of infectious disease kits to meet your routine assay requirements, then block scientific can make it available for you. as a well known lab equipment dealer with many years experience in the industry, block scientific has always focused on meeting even the most challenging client requirements. we offer the hbc ab screening, 96 wells at an affordable price.
hepatitis b elisa
– for quality antibody determination
this product is a competitive enzyme immuno assay (elisa) for the determination of antibodies to hepatitis b core antigen in human plasma and sera.
the assay is based on the principle of competition where the antibodies in the sample compete with a monoclonal antibody for a fixed amount of antigen on the solid phase.
a purified recombinant hbcag is coated to the microwells.
the donor’s serum/plasma is added to the microwell together with an additive able to block interferences present in the sample.
in the second incubation after washing, a monoclonal antibody, conjugated with horseradish peroxidase (hrp) and specific for hbcag is added and binds to the free rec-hbcag coated on the plastic.
after incubation, microwells are washed to remove any unbound conjugate and then the chromogen/substrate is added. in the presence of peroxidase enzyme the colorless substrate is hydrolyzed to a colored end-product.
the color intensity is inversely proportional to the amount of antibodies to hbcag present in the sample.
for research use only
- kit components – microplate, positive control, negative control, calibrator, enzyme conjugate, wash buffer concentrate, chromogen/substrate, sulphuric acid, specimen diluent, plate sealing foil, instruction manual
- a study conducted on an opened kit has not pointed out any relevant loss of activity up to 6 re-uses of the device and up to 6 months.
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