fasciola elisa, 96 wells
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- drg international inc.
- fasciola elisa, 96 wells
if you are in search of infectious disease kits to meet your routine assay requirements, then block scientific can make it available for you. as a well known lab equipment dealer with many years experience in the industry, block scientific has always focused on meeting even the most challenging client requirements. we offer the fasciola elisa, 96 wells at an affordable price.
drg fasciola igg enzyme immunoassay kit
– solid phase elisa
the drg fasciola igg elisa kit is a solid phase enzyme-linked immunosorbent assay (elisa). it provides materials for the qualitative and semiquantitative determination of igg-class antibodies to fasciola in serum.
fasciola, also known as the common liver fluke or sheep liver fluke, is a parasitic flatworm of the class trematoda, phylum platyhelminthes that infects liver of a various mammals, including human. the disease caused by the fluke is called fascioliasis (also known as fasciolosis). fasciola is world-wide distributed and causes great economic losses in sheep and cattle.
microtiter wells as a solid phase are coated with fasciola antigen from adult liver fluke.
diluted patient specimens and ready-for-use controls are pipetted into these wells. during incubation fasciolaspecific antibodies of positive specimens and controls are bound to the immobilized antigens.
after a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human igg antibodies are dispensed into the wells. during a second incubation this anti-igg conjugate binds specifically to igg antibodies resulting in the formation of enzyme-linked immune complexes.
after a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with tmb substrate and development of a blue color. the blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid.
the intensity of this color is directly proportional to the amount of fasciola-specific igg antibody in the patient specimen. absorbance at 450 nm is read using an elisa microtiter plate reader.
for in vitro use only
- reagents provided – sample diluent, microtiterwells, low control, high control, enzyme conjugate, calibrator, stop solution, substrate solution, wash solution
- storage – when stored at 2 °c to 8 °c unopened reagents will retain reactivity until expiration date.
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