Epstein-Barr Virus (EBNA-1) IgM ELISA, 96 Wells

To add this product to your interest list, sign up or login.

Epstein-Barr Virus (EBNA-1) IgM ELISA, 96 Wells

Manufacturer: DRG International Inc.
Model: Epstein-Barr Virus (EBNA-1) IgM ELISA, 96 wells
Product Code: EIA4247

Buy Now

The Epstein-Barr Virus (EBNA-1) IgM ELISA, 96 wells is one among many essential infectious disease kits available at Block Scientific. We have a wide range of laboratory supplies and equipment from popular manufacturers in the industry. We are one of the leading laboratory equipment dealers in Bellport, New York.

Epstein Barr Virus ELISA

– Useful and Reliable

The DRG Epstein-Barr Virus (EBNA-1) IgM Enzyme Immunoassay Kit is a useful and reliable kit for measurement of IgMclass antibodies to Epstein-Barr virus nuclear-1 antigen (EBV-EBNA-1) in serum.

Test Principle

The DRG Epstein-Barr Virus (EBNA-1) IgM ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA).

Patient samples are diluted with Sample Diluent and additionally incubated with IgG-RF-Sorbent, containing hyper-immune anti-human IgG-class antibody to eliminate competitive inhibition from specific IgG and to remove rheumatoid factors. This pretreatment avoids false negative or false positive results.

Microtiter wells as a solid phase are coated with recombinant EBV nuclear-1 antigen.

Diluted patient specimens and ready-for-use controls are pipetted into these wells. During incubation EBNA-1-specific antibodies of positive specimens and controls are bound to the immobilized antigens.

After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgM antibodies are dispensed into the wells. During a second incubation this anti-IgM conjugate binds specifically to IgM antibodies resulting in the formation of enzyme-linked immune complexes.

After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid.

The intensity of this color is directly proportional to the amount of EBNA-1-specific IgM antibody in the patient specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.

Features

  • Kit Contents – sample diluent, microtiterwells, IgG-RF-Sorbent, low control, high control, substrate solution, enzyme conjugate, wash solution, stop solution, calibrator
  • Storage – When stored at 2 °C to 8 °C unopened reagents will retain reactivity until expiration date.

For more information about the Epstein-Barr Virus (EBNA-1) IgM ELISA, 96 wells and other infectious disease kits, visit our online store. For more information about our product line, dial 631-589-1118 or send an email to info@blockscientific.com.