epstein-barr virus (ebna-1) igm elisa, 96 wells
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- drg international inc.
the epstein-barr virus (ebna-1) igm elisa, 96 wells is one among many essential infectious disease kits available at block scientific. we have a wide range of laboratory supplies and equipment from popular manufacturers in the industry. we are one of the leading laboratory equipment dealers in bellport, new york.
epstein barr virus elisa
– useful and reliable
the drg epstein-barr virus (ebna-1) igm enzyme immunoassay kit is a useful and reliable kit for measurement of igmclass antibodies to epstein-barr virus nuclear-1 antigen (ebv-ebna-1) in serum.
the drg epstein-barr virus (ebna-1) igm elisa kit is a solid phase enzyme-linked immunosorbent assay (elisa).
patient samples are diluted with sample diluent and additionally incubated with igg-rf-sorbent, containing hyper-immune anti-human igg-class antibody to eliminate competitive inhibition from specific igg and to remove rheumatoid factors. this pretreatment avoids false negative or false positive results.
microtiter wells as a solid phase are coated with recombinant ebv nuclear-1 antigen.
diluted patient specimens and ready-for-use controls are pipetted into these wells. during incubation ebna-1-specific antibodies of positive specimens and controls are bound to the immobilized antigens.
after a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human igm antibodies are dispensed into the wells. during a second incubation this anti-igm conjugate binds specifically to igm antibodies resulting in the formation of enzyme-linked immune complexes.
after a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with tmb substrate and development of a blue color. the blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid.
the intensity of this color is directly proportional to the amount of ebna-1-specific igm antibody in the patient specimen. absorbance at 450 nm is read using an elisa microtiter plate reader.
- kit contents – sample diluent, microtiterwells, igg-rf-sorbent, low control, high control, substrate solution, enzyme conjugate, wash solution, stop solution, calibrator
- storage – when stored at 2 °c to 8 °c unopened reagents will retain reactivity until expiration date.
for more information about the epstein-barr virus (ebna-1) igm elisa, 96 wells and other infectious disease kits, visit our online store. for more information about our product line, dial 631-589-1118 or send an email to email@example.com.