Epstein-Barr Virus (EBNA-1) IgG ELISA, 96 Wells

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Epstein-Barr Virus (EBNA-1) IgG ELISA, 96 Wells

Manufacturer: DRG International Inc.
Model: Epstein Barr Virus VCA IgG, 96 wells
Product Code: EIA4246

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The Epstein-Barr Virus (EBNA-1) IgG ELISA, 96 wells is one among many essential infectious disease kits available at Block Scientific. We have a wide range of laboratory supplies and equipment from popular manufacturers in the industry. We are one of the leading laboratory equipment dealers in Bellport, New York.

Epstein Barr Virus ELISA

– Useful and Reliable

This product is a useful and reliable kit for the qualitative and semiquantitative determination of IgG-class antibodies to Epstein-Barr virus nuclear antigen type 1 (EBV-EBNA-1) in serum.

Test Principle

The DRG Epstein-Barr Virus (EBNA-1) IgG ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) Microtiter wells as a solid phase are coated with recombinant EBV nuclear antigen type 1.

Diluted patient specimens and ready-for-use controls are pipetted into these wells. During incubation EBNA-1-specific antibodies of positive specimens and controls are bound to the immobilized antigens.

After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgG antibodies are dispensed into the wells. During a second incubation this anti-IgG conjugate binds specifically to IgG antibodies resulting in the formation of enzyme-linked immune complexes.

After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid.

The intensity of this color is directly proportional to the amount of EBNA-1-specific IgG antibody in the patient specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.

Features

  • Kit Contents – sample diluent, microtiterwells, low control, high control,
    cut-off control, substrate solution, enzyme conjugate, wash solution, stop solution
  • Storage – When stored at 2 °C to 8 °C unopened reagents will retain reactivity until expiration date.

For more information about the Epstein-Barr Virus (EBNA-1) IgG ELISA, 96 wells and other infectious disease kits, visit our online store. For more information about our product line, dial 631-589-1118 or send an email to info@blockscientific.com.