e. coli verotoxin 1+2 ag (stool) elisa, 96 wells
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- drg international inc.
the drg e. coli verotoxin 1+2 ag elisa, 96 wells is an in vitro device for direct detection of verotoxin 1 and 2 (shiga-toxin 1 and 2) in faecal specimens and stool culture supernatants. to purchase this product at a great price and have it promptly delivered to you, you have come to the right place. we are block scientific, one of the leading laboratory equipment suppliers in the u.s.
e coli verotoxin elisa
the drg e. coli verotoxin 1+2 ag elisa is an indirect two-site-immunoassay for the qualitative determination of verotoxin 1 and 2 based on polyclonal and monoclonal antibodies.
verotoxin 1 and/or 2 of specimens and the positive control react with polyclonal anti-verotoxin 1 and 2 antibodies coated on the solid phase of the microplate. after incubation for 60 minutes at 22-25°c non-bound material is removed by a washing step.
subsequently bound toxins specifically react with biotinylated monoclonal anti-verotoxin 1 and anti-verotoxin 2 antibodies during a second incubation period of 30 min at 22-25°c. non-bound material is separated from the solid-phase immune complexes by a subsequent washing step.
during the next incubation period of 30 min at 22-25°c horseradish peroxidase (hrp) conjugated streptavidin reacts with the bound biotinylated antibodies. unbound conjugate is removed by a washing step.
test components – microtitration plate, sample diluent for verotoxin 1+2, wash buffer, 10-fold, negative control, streptavidin-poly-hrp-conjugate, biotin-conjugate, stop solution, substrate storage – after opening, all kit components are stable for at least 2 months, provided proper storage (2-8°c, preferably in the original kit box).
if you have any questions about the e. coli verotoxin 1+2 ag (stool) elisa, 96 wells or other infectious disease kits, visit our online store. for more information about our product line, dial 631-589-1118 and speak to one of our customer service representatives or send an email to firstname.lastname@example.org.