E. Coli Verotoxin 1+2 Ag (Stool) ELISA, 96 Wells

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E. Coli Verotoxin 1+2 Ag (Stool) ELISA, 96 Wells

Manufacturer: DRG International Inc.
Model: E. Coli Verotoxin 1+2 Ag (stool) ELISA, 96 wells
Product Code: EIA4452

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The DRG E. coli Verotoxin 1+2 Ag ELISA, 96 wells is an in vitro device for direct detection of verotoxin 1 and 2 (shiga-toxin 1 and 2) in faecal specimens and stool culture supernatants. To purchase this product at a great price and have it promptly delivered to you, you have come to the right place. We are Block Scientific, one of the leading laboratory equipment suppliers in the U.S.

E Coli Verotoxin Elisa

– Two-Site-Immunoassay

The DRG E. coli Verotoxin 1+2 Ag ELISA is an indirect two-site-immunoassay for the qualitative determination of verotoxin 1 and 2 based on polyclonal and monoclonal antibodies.

Test Principle

Verotoxin 1 and/or 2 of specimens and the positive control react with polyclonal anti-verotoxin 1 and 2 antibodies coated on the solid phase of the microplate. After incubation for 60 minutes at 22-25°C non-bound material is removed by a washing step.

Subsequently bound toxins specifically react with biotinylated monoclonal anti-verotoxin 1 and anti-verotoxin 2 antibodies during a second incubation period of 30 min at 22-25°C. Non-bound material is separated from the solid-phase immune complexes by a subsequent washing step.

During the next incubation period of 30 min at 22-25°C horseradish peroxidase (HRP) conjugated streptavidin reacts with the bound biotinylated antibodies. Unbound conjugate is removed by a washing step.

Features

Test Components – Microtitration plate, Sample diluent for Verotoxin 1+2, Wash buffer, 10-fold, Negative control, Streptavidin-poly-HRP-conjugate, Biotin-conjugate, Stop solution, Substrate Storage – After opening, all kit components are stable for at least 2 months, provided proper storage (2-8°C, preferably in the original kit box).

If you have any questions about the E. Coli Verotoxin 1+2 Ag (stool) ELISA, 96 wells or other infectious disease kits, visit our online store. For more information about our product line, dial 631-589-1118 and speak to one of our customer service representatives or send an email to info@blockscientific.com.