cmv igg elisa, 96 wells
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- drg international inc.
- cmv igg elisa, 96 wells
the drg cytomegalie virus (cmv) igg elisa, 96 wells provides materials for measurement of iggclass antibodies to cytomegalie virus (cmv) in serum. to purchase this product at a great price and have it promptly delivered to you, you have come to the right place. we are block scientific, one of the leading laboratory equipment suppliers in the u.s.
drg cytomegalie virus (cmv) igg elisa kit
– solid phase elisa
the drg cytomegalie virus (cmv) igg elisa kit is a solid phase enzyme-linked immunosorbent assay (elisa).
microtiter wells as a solid phase are coated with cytomegalie virus (cmv) antigen. diluted patient specimens and ready-for-use controls are pipetted into these wells. during incubation cytomegalie virus (cmv)-specific antibodies of positive specimens and controls are bound to the immobilized antigens.
after a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human igg antibodies are dispensed into the wells. during a second incubation this anti-igg conjugate binds specifically to igg antibodies resulting in the formation of enzyme-linked immune complexes.
after a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with tmb substrate and development of a blue color. the blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid.
the intensity of this color is directly proportional to the amount of cytomegalie virus (cmv)-specific igg antibody in the patient specimen. absorbance at 450 nm is read using an elisa microtiter plate reader.
in the united states, this kit is intended for research use only.
- kit components – sample diluent, microtiterwells, standard (standard 1 – 3)*, neg. control, pos. control, substrate solution, enzyme conjugate, wash solution, stop solution
- storage – when stored at 2 °c to 8 °c unopened reagents will retain reactivity until expiration date.
if you have any questions about the cmv igg elisa, 96 wells or other infectious disease kits, visit our online store. for more information about our product line, dial 631-589-1118 and speak to one of our customer service representatives or send an email to email@example.com.