Chlamydia Trachomatis IgM ELISA, 96 Wells
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Manufacturer: DRG International Inc.
Model: Chlamydia trachomatis IgM ELISA, 96 wells
Product Code: EIA3463
The DRG Chlamydia trachomatis IgM ELISA, 96 wells provides materials for the qualitative and semiquantitative determination of IgM-class antibodies to Chlamydia trachomatis in serum. You can get this product for a great price from Block Scientific, one of the leading lab equipment dealers in Bellport, New York.
Enzyme Immunoassay Kit
This product is a quality solid phase enzyme-linked immunosorbent assay (ELISA). You can conveniently use it for your in vitro requirements.
This assay is intended for in vitro use only. In the United States, this kit is intended for Research Use Only.
- Provided reagents: microtiterwells, high control, low control, IgG-RF-Sorbent, sample diluent, substrate solution, enzyme conjugate, calibrator, wash solution, stop solution
- Incubation time: 60/30/15 min
Principle of The Test
Patient samples are diluted with Sample Diluent and additionally incubated with IgG-RF-Sorbent, containing hyperimmune anti-human IgG-class antibody to eliminate competitive inhibition from specific IgG and to remove rheumatoid factors. This pretreatment avoids false negative or false positive results. Microtiter wells as a solid phase are coated with Chlamydia trachomatis antigen. Diluted patient specimens and ready-for-use controls are pipetted into these wells. During incubation Chlamydia trachomatis-specific antibodies of positive specimens and controls are bound to the immobilized antigens. After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgM antibodies are dispensed into the wells. During a second incubation this anti-IgM conjugate binds specifically to IgM antibodies resulting in the formation of enzyme-linked immune complexes. After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directly proportional to the amount of Chlamydia trachomatis-specific IgM antibody in the patient specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.
For convenient online purchase of the Chlamydia trachomatis IgM ELISA, 96 wells and other infectious disease kits, visit our online store. If you have any questions about the product, we would be happy to answer them for you. Dial 631-589-1118 or send an email to email@example.com.