Chlamydia Pneumoniae IgM ELISA, 96 Wells
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Manufacturer: DRG International Inc.
Model: Chlamydia pneumoniae IgM ELISA, 96 wells
Product Code: EIA3913
The DRG Chlamydia pneumoniae IgM-ELISA, 96 wells is intended for the qualitative determination of IgM class antibodies against Chlamydia pneumoniae in human serum or plasma (citrate).You can get this product for a great price from Block Scientific, one of the leading lab equipment dealers in Bellport, New York.
Chlamydia Pneumoniae IgM
– Useful Enzyme Immunoassay Kit
This product is a quality, useful infectious disease kit based on the ELISA techniques (Enzyme-linked Immunosorbent Assay). You can conveniently use it for your in vitro requirements.
This kit is intended for Research Use Only. Not for use in diagnostic procedures.
Provided reagents: IgG Sample Diluent, Chlamydia pneumoniae Coated Wells (IgG), washing solution (20x conc.), stop solution, TMB substrate solution, Chlamydia pneumoniae IgG Cut-off Control, Chlamydia pneumoniae IgG Positive Control, Chlamydia pneumoniae IgG Negative Control, Chlamydia pneumoniae anti-IgG Conjugate
- Incubation: 1 h 37 C / 30′, 15′ RT
- Stand.Range/Sensitivity: pos. + neg. control
- Specimen: 10 ul serum or plasma
- Type: Qualitative
PRINCIPLE OF THE ASSAY
The qualitative immunoenzymatic determination of IgM-class antibodies against Chlamydia pneumoniae is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiter strip wells are precoated with Chlamydia pneumoniae antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled antihuman IgM conjugate is added. This conjugate binds to the captured Chlamydia pneumoniae-specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of Chlamydia pneumoniaespecific IgM antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint color. Absorbance at 450 nm is read using an ELISA microwell plate reader.
Chlamydiae are no motile, Gram negative and obligatory intracellular growing bacteria which form characteristic inclusions within the cytoplasm of parasitized cells. They are easily visible in the light microscope. Three different Chlamydia species pathogenic for humans are known: Chlamydia trachomatis, Chlamydia pneumoniae and Chlamydia psittaci, and one species only pathogenic for animals (C. pecorum). Chlamydia trachomatis is the most prevalent agent of sexually transmitted diseases worldwide (400-500 million cases) and the number of infections is constantly growing. Pregnant women infected with C. trachomatis may transmit these bacteria during childbirth, causing conjunctivitis or pneumonia in newborns. Untreated cases of chlamydial infection can lead to chronic salpingitis, possibly resulting in ectopic pregnancy or infertility. In males, C. trachomatis is a major cause of non-gonococcal urethritis. A severe problem in Chlamydia infections is the frequent asymptomatic insidious course which may result in the initiation of chronic diseases. In many instances primary infections are not recognized and only the sequelae caused by ascended, persisting agents are diagnosed.
For convenient online purchase of the Chlamydia pneumoniae IgM ELISA, 96 wells and other infectious disease kits, visit our online store. If you have any questions about the product, we would be happy to answer them for you. Dial 631-589-1118 or send an email to email@example.com.