candida albicans igm elisa, 96 wells
to add this product to your interest list, sign up or login.
- drg international inc.
if you are looking to purchase the candida albicans igm elisa, 96 wells, you have come to the right place. we are block scientific, an established laboratory equipment supplier with no less than three decades of experience in dealing in new and refurbished lab equipment. this product is just one of many infectious disease kits from drg international inc. in our inventory.
the drg candida albicans igm enzyme immunoassay kit provides materials for the qualitative and semiquantitative determination of igm-class antibodies to candida albicans in serum. this assay is intended for in vitro use only.
- incubation: 60 min 37 c; 30 / 15 min. rt
- stand.range/sensitivity: pos. / neg. control
- specimen: 10 ul serum (1+100 diluted)
- type: qualitative
- method: mtpl
the drg candida albicans igm elisa kit is a solid phase enzyme-linked immunosorbent assay (elisa). patient samples are diluted with sample diluent and additionally incubated with igg-rf-sorbent, containing hyperimmune anti-human igg-class antibody to eliminate competitive inhibition from specific igg and to remove rheumatoid factors. this pretreatment avoids false negative or false positive results. microtiter wells as a solid phase are coated with candida albicans antigen.
pretreated patient specimens and ready-for-use controls are pipetted into these wells. during incubation candida albicans-specific antibodies of positive specimens and controls are bound to the immobilized antigens. after a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human igm antibodies are dispensed into the wells. during a second incubation this anti-igm conjugate binds specifically to igm antibodies resulting in the formation of enzyme-linked immune complexes. after a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with tmb substrate and development of a blue color. the blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. the intensity of this color is directly proportional to the amount of candida albicans-specific igm antibody in the patient specimen. absorbance at 450 nm is read using an elisa microtiter plate reader.
for convenient online purchase of the candida albicans igm elisa, 96 wells and other infectious disease kits, visit our online store. for more information about the product, dial 631-589-1118 or send an email to email@example.com.