bordetella pertussis/toxin igm elisa, 96 wells
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- drg international inc.
the bordetella pertussis/toxin igm elisa, 96 wells is just one of a wide range of infectious disease kits available with us for an awesome price. we are block scientific, a popular laboratory equipment supplier strategically situated in bellport, new york and with national and international clients.
the drg bordetella pertussis / toxin igm enzyme immunoassay kit provides materials for the qualitative and semiquantitative determination of igm-class antibodies to bordetella pertussis and bordetella pertussis toxin in serum. this assay is intended for in vitro use only. in the united states, this kit is intended for research use only.
kit components – sample diluent, microtiterwells, low control, high control, igg-rf-sorbent, enzyme conjugate, calibrator, stop solution, substrate solution, wash solution
specimen – serum may be utilized in this assay
storage – when stored at 2 °c to 8 °c, unopened reagents will retain reactivity until expiration date. opened reagents must be stored at 2 °c to 8 °c.
principle of the test
the drg bordetella pertussis / toxin igm elisa kit is a solid phase enzyme-linked immunosorbent assay (elisa) patient samples are diluted with sample diluent and additionally incubated with igg-rf-sorbent, containing hyperimmune anti-human igg-class antibody to eliminate competitive inhibition from specific igg and to remove rheumatoid factors. this pretreatment avoids false negative or false positive results. microtiter wells as a solid phase are coated with bordetella pertussis and bordetella pertussis toxin antigen. pretreated patient specimens and ready-for-use controls are pipetted into these wells. during incubation bordetella pertussis and bordetella pertussis toxin-specific antibodies of positive specimens and controls are bound to the immobilized antigens. after a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human igm antibodies are dispensed into the wells. during a second incubation this anti-igm conjugate binds specifically to igm antibodies resulting in the formation of enzyme-linked immune complexes.
after a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with tmb substrate and development of a blue color. the blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. the intensity of this color is directly proportional to the amount of bordetella pertussis and bordetella pertussis toxinspecific igm antibody in the patient specimen. absorbance at 450 nm is read using an elisa microtiter plate reader.