bordetella pertussis/toxin igg elisa, 96 wells
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- drg international inc.
the drg bordetella pertussis/toxin igg elisa, 96 wells provides materials for the qualitative and semiquantitative determination of igg-class antibodies to bordetella pertussis and bordetella pertussis toxin in serum. this assay is intended for in vitro diagnostic use only. get this product at an amazing price from block scientific, your reliable supplier for all kinds of new and refurbished laboratory equipment including infectious disease kits.
enzyme immunoassay kit
this product is a quality enzyme immunoassay kit, a solid phase enzyme-linked immunosorbent assay (elisa).
- kit contents – microtiterwells, positive control, negative control, sample diluent, cut-off control, enzyme conjugate, stop solution, substrate solution, wash solution
- unopened reagents will retain reactivity till date of expiry if stored at 2 °c to 8 °c
microtiter wells as a solid phase are coated with bordetella pertussis and bordetella pertussis toxin antigen. diluted patient specimens and ready-for-use controls are pipetted into these wells. during incubation bordetella pertussis and bordetella pertussis toxin-specific antibodies of positive specimens and controls are bound to the immobilized antigens. after a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human igg antibodies are dispensed into the wells. during a second incubation this anti-igg conjugate binds specifically to igg antibodies resulting in the formation of enzyme-linked immune complexes. after a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with tmb substrate and development of a blue color. the blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. the intensity of this color is directly proportional to the amount of bordetella pertussis and bordetella pertussis toxinspecific igg antibody in the patient specimen. absorbance at 450 nm is read using an elisa microtiter plate reader.