bordetella pertussis/toxin iga elisa, 96 wells
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- drg international inc.
the drg bordetella pertussis / toxin iga elisa, 96 wells provides materials for the qualitative and semiquantitative determination of iga-class antibodies to bordetella pertussis and bordetella pertussis toxin in serum. this assay is intended for in vitro use only. in the united states, this kit is intended for research use only. block scientific, a popular laboratory equipment supplier in bellport, new york can provide this product to you at an affordable price.
enzyme-linked immunosorbent assay
this product is a solid phase enzyme-linked immunosorbent assay (elisa).
- unopened reagents will retain reactivity till date of expiry if stored at 2 °c to 8 °c
- kit contents – microtiterwells, igg-rf-sorbent, sample diluent, low control, high control, substrate solution, enzyme conjugate, stop solution, substrate solution, wash solution
not to be used in diagnostic procedures
principle of the test
microtiter wells as a solid phase are coated with bordetella pertussis and bordetella pertussis toxin antigen. diluted patient specimens and ready-for-use controls are pipetted into these wells. during incubation bordetella pertussis and bordetella pertussis toxin-specific antibodies of positive specimens and controls are bound to the immobilized antigens. after a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human iga antibodies are dispensed into the wells. during a second incubation this anti-iga conjugate binds specifically to iga antibodies resulting in the formation of enzyme-linked immune complexes. after a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with tmb substrate and development of a blue color. the blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. the intensity of this color is directly proportional to the amount of bordetella pertussis and bordetella pertussis toxinspecific iga antibody in the patient specimen. absorbance at 450 nm is read using an elisa microtiter plate reader.
to buy online
for convenient online purchase of the drg bordetella pertussis / toxin iga elisa, 96 wells and other infectious disease kits, visit our online store. dial 631-589-1118 or send an email to email@example.com if you want more information about this product.