astrovirus (stool) elisa, 96 wells
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- drg international inc.
- astrovirus (stool) elisa, 96 wells
astrovirus (stool) elisa, 96 wells is for the detection of astrovirus in faecal samples. to purchase this value product at an affordable price, you have come to the right place. we are block scientific, a well-known laboratory equipment supplier based in bellport, new york but serving clients situated both within the u.s. as well as outside of it.
astrovirus ag elisa is a one-step enzyme immunoassay on the basis of polyclonal and monoclonal antibodies against astrovirus antigens.
diluted stool specimens and horseradish peroxidase (hrp) labelled monoclonal anti-astrovirus-antibodies are dispensed simultaneously into the wells of a microtitration plate coated with polyclonal anti-astrovirus-antibodies. after an incubation time of 60 min at room temperature (rt) unbound components are removed by a washing step. hrp converts the subsequently added colorless substrate solution of 3,3′,5,5′-tetramethylbenzidine (tmb) within a 10 min reaction time at room temperature protected from light into a blue product. the enzyme reaction is terminated by sulphuric acid dispensed into the wells turning the solution from blue to yellow.
the optical density (od) of the solution read at 450/=620 nm is directly proportional to the specifically bound amount of astrovirus.
- test components – microtitration plate, 12 single breakable 8-well strips
(in all 96 wells) coated with polyclonal anti-astrovirusantibodies (rabbit); sample diluent, wash buffer, 10-fold; low control, high control, substrate, hrp-conjugate, stop solution
- result interpretation
cut-off determination: od low control + 0.10
samples with od values equal with or higher than the cut-off are considered high while those with od values below the cut-off are considered low for astrovirus antigen.
a complete list of the infectious disease kits we provide is available on our online store. to know more about any of these kits including the astrovirus (stool) elisa, 96 wells, please dial 631-589-1118 or send an email to firstname.lastname@example.org.